Microvillar dynamic in renal tubular epithelial cells mediated by insulin/PLCγ signal pathway

Lida Wu; Tongri Liu; Yuchun Gu

doi:10.1016/j.bbrc.2020.10.046

Microvillar dynamic in renal tubular epithelial cells mediated by insulin/PLCγ signal pathway

Lida Wu
, Tongri Liu
, Yuchun Gu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Significant cellular morphology changes in renal tubules were observed in diabetes patients and animal models. However, the interaction between insulin and tubular epithelial cells microvillar structure remains obscure. To understand microvillar dynamics, we used Scanning Ion Conductance Microscope to visualize microvillar in the living cell. Here, we found two layers of microvilli on the tubular epithelial cell surface: short compact microvilli and netlike long microvilli. Insulin treatment could increase microvilli length and density. This process was mediated by the PI₃K/PLCγ signaling pathway, other than the PI₃K/Arp2/3 signal pathway. In conclusion, our findings present a novel insulin signaling transduction mechanism, which contributes to understanding renal tubular epithelial cell microvilli dynamic regulation.

Original language	English
Pages (from-to)	1020-1025
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	534
Early online date	31 Oct 2020
DOIs	https://doi.org/10.1016/j.bbrc.2020.10.046
Publication status	Published - 1 Jan 2021

Funding

This work is supported by the National Basic Research Program of China (973 Program) ( 2013CB531206 and 2012CB517803 ), the National Natural Science Foundation of China ( 81170236 and 31127001 ).

Keywords

Insulin signal transduction
Microvilli formation
Renal tubular epithelial cells

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title = "Microvillar dynamic in renal tubular epithelial cells mediated by insulin/PLCγ signal pathway",

abstract = "Significant cellular morphology changes in renal tubules were observed in diabetes patients and animal models. However, the interaction between insulin and tubular epithelial cells microvillar structure remains obscure. To understand microvillar dynamics, we used Scanning Ion Conductance Microscope to visualize microvillar in the living cell. Here, we found two layers of microvilli on the tubular epithelial cell surface: short compact microvilli and netlike long microvilli. Insulin treatment could increase microvilli length and density. This process was mediated by the PI3K/PLCγ signaling pathway, other than the PI3K/Arp2/3 signal pathway. In conclusion, our findings present a novel insulin signaling transduction mechanism, which contributes to understanding renal tubular epithelial cell microvilli dynamic regulation.",

keywords = "Insulin signal transduction, Microvilli formation, Renal tubular epithelial cells",

author = "Lida Wu and Tongri Liu and Yuchun Gu",

year = "2021",

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doi = "10.1016/j.bbrc.2020.10.046",

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T1 - Microvillar dynamic in renal tubular epithelial cells mediated by insulin/PLCγ signal pathway

AU - Wu, Lida

AU - Liu, Tongri

AU - Gu, Yuchun

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Significant cellular morphology changes in renal tubules were observed in diabetes patients and animal models. However, the interaction between insulin and tubular epithelial cells microvillar structure remains obscure. To understand microvillar dynamics, we used Scanning Ion Conductance Microscope to visualize microvillar in the living cell. Here, we found two layers of microvilli on the tubular epithelial cell surface: short compact microvilli and netlike long microvilli. Insulin treatment could increase microvilli length and density. This process was mediated by the PI3K/PLCγ signaling pathway, other than the PI3K/Arp2/3 signal pathway. In conclusion, our findings present a novel insulin signaling transduction mechanism, which contributes to understanding renal tubular epithelial cell microvilli dynamic regulation.

AB - Significant cellular morphology changes in renal tubules were observed in diabetes patients and animal models. However, the interaction between insulin and tubular epithelial cells microvillar structure remains obscure. To understand microvillar dynamics, we used Scanning Ion Conductance Microscope to visualize microvillar in the living cell. Here, we found two layers of microvilli on the tubular epithelial cell surface: short compact microvilli and netlike long microvilli. Insulin treatment could increase microvilli length and density. This process was mediated by the PI3K/PLCγ signaling pathway, other than the PI3K/Arp2/3 signal pathway. In conclusion, our findings present a novel insulin signaling transduction mechanism, which contributes to understanding renal tubular epithelial cell microvilli dynamic regulation.

KW - Insulin signal transduction

KW - Microvilli formation

KW - Renal tubular epithelial cells

UR - https://www.scopus.com/pages/publications/85094833261

UR - https://www.sciencedirect.com/science/article/pii/S0006291X20319641?via%3Dihub

U2 - 10.1016/j.bbrc.2020.10.046

DO - 10.1016/j.bbrc.2020.10.046

M3 - Article

AN - SCOPUS:85094833261

SN - 0006-291X

VL - 534

SP - 1020

EP - 1025

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

Microvillar dynamic in renal tubular epithelial cells mediated by insulin/PLCγ signal pathway

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