Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans

A.J. Wadley, Y.W. Chen, S.J. Bennett, G.Y.H. Lip, J.E. Turner, J.P. Fisher, S. Aldred*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Introduction. Peroxiredoxin (PRDX) and thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods. Healthy males (n = 10, mean ± SD: 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at moderate (60% VO2MAX; 27 min, MOD) and high (80% VO2MAX; 20 min, HIGH) intensities, and a low-volume high-intensity interval training trial (10 × 1 min 90% VO2MAX, LV-HIIT). Peripheral blood mononuclear cells were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during, and 30 min following exercise (post + 30). The activities of TRX reductase (TRX-R) and the nuclear factor kappa B (NF-κB) p65 subunit were also assessed. Results. TRX-1 increased during exercise in all trials (MOD, + 84.5%; HIGH, + 64.1%; LV-HIIT, + 205.7%; p < 05), whereas over-oxidised PRDX increased during HIGH only (MOD, - 28.7%; HIGH, + 202.9%; LV-HIIT, - 22.7%; p < .05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p < 0.05). Discussion. All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high-intensity exercise and the mechanisms of PRDX-regulatory control.

Original languageEnglish
Pages (from-to)290-298
Number of pages9
JournalFree Radical Research
Volume49
Issue number3
Early online date4 Feb 2015
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

Peroxiredoxins
Thioredoxins
Monitoring
NF-kappa B
Oxidation-Reduction
Thioredoxin-Disulfide Reductase
Cell signaling
Proteins
Peroxides
Blood Cells
Protein Isoforms
Blood
Antioxidants
Oxidation

Keywords

  • antioxidant
  • cytokines
  • exercise
  • inflammation
  • Redox status

Cite this

Wadley, A. J., Chen, Y. W., Bennett, S. J., Lip, G. Y. H., Turner, J. E., Fisher, J. P., & Aldred, S. (2015). Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans. Free Radical Research, 49(3), 290-298. https://doi.org/10.3109/10715762.2014.1000890
Wadley, A.J. ; Chen, Y.W. ; Bennett, S.J. ; Lip, G.Y.H. ; Turner, J.E. ; Fisher, J.P. ; Aldred, S. / Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans. In: Free Radical Research. 2015 ; Vol. 49, No. 3. pp. 290-298.
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abstract = "Introduction. Peroxiredoxin (PRDX) and thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods. Healthy males (n = 10, mean ± SD: 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at moderate (60{\%} VO2MAX; 27 min, MOD) and high (80{\%} VO2MAX; 20 min, HIGH) intensities, and a low-volume high-intensity interval training trial (10 × 1 min 90{\%} VO2MAX, LV-HIIT). Peripheral blood mononuclear cells were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during, and 30 min following exercise (post + 30). The activities of TRX reductase (TRX-R) and the nuclear factor kappa B (NF-κB) p65 subunit were also assessed. Results. TRX-1 increased during exercise in all trials (MOD, + 84.5{\%}; HIGH, + 64.1{\%}; LV-HIIT, + 205.7{\%}; p < 05), whereas over-oxidised PRDX increased during HIGH only (MOD, - 28.7{\%}; HIGH, + 202.9{\%}; LV-HIIT, - 22.7{\%}; p < .05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p < 0.05). Discussion. All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high-intensity exercise and the mechanisms of PRDX-regulatory control.",
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Wadley, AJ, Chen, YW, Bennett, SJ, Lip, GYH, Turner, JE, Fisher, JP & Aldred, S 2015, 'Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans', Free Radical Research, vol. 49, no. 3, pp. 290-298. https://doi.org/10.3109/10715762.2014.1000890

Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans. / Wadley, A.J.; Chen, Y.W.; Bennett, S.J.; Lip, G.Y.H.; Turner, J.E.; Fisher, J.P.; Aldred, S.

In: Free Radical Research, Vol. 49, No. 3, 01.03.2015, p. 290-298.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans

AU - Wadley, A.J.

AU - Chen, Y.W.

AU - Bennett, S.J.

AU - Lip, G.Y.H.

AU - Turner, J.E.

AU - Fisher, J.P.

AU - Aldred, S.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Introduction. Peroxiredoxin (PRDX) and thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods. Healthy males (n = 10, mean ± SD: 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at moderate (60% VO2MAX; 27 min, MOD) and high (80% VO2MAX; 20 min, HIGH) intensities, and a low-volume high-intensity interval training trial (10 × 1 min 90% VO2MAX, LV-HIIT). Peripheral blood mononuclear cells were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during, and 30 min following exercise (post + 30). The activities of TRX reductase (TRX-R) and the nuclear factor kappa B (NF-κB) p65 subunit were also assessed. Results. TRX-1 increased during exercise in all trials (MOD, + 84.5%; HIGH, + 64.1%; LV-HIIT, + 205.7%; p < 05), whereas over-oxidised PRDX increased during HIGH only (MOD, - 28.7%; HIGH, + 202.9%; LV-HIIT, - 22.7%; p < .05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p < 0.05). Discussion. All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high-intensity exercise and the mechanisms of PRDX-regulatory control.

AB - Introduction. Peroxiredoxin (PRDX) and thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods. Healthy males (n = 10, mean ± SD: 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at moderate (60% VO2MAX; 27 min, MOD) and high (80% VO2MAX; 20 min, HIGH) intensities, and a low-volume high-intensity interval training trial (10 × 1 min 90% VO2MAX, LV-HIIT). Peripheral blood mononuclear cells were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during, and 30 min following exercise (post + 30). The activities of TRX reductase (TRX-R) and the nuclear factor kappa B (NF-κB) p65 subunit were also assessed. Results. TRX-1 increased during exercise in all trials (MOD, + 84.5%; HIGH, + 64.1%; LV-HIIT, + 205.7%; p < 05), whereas over-oxidised PRDX increased during HIGH only (MOD, - 28.7%; HIGH, + 202.9%; LV-HIIT, - 22.7%; p < .05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p < 0.05). Discussion. All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high-intensity exercise and the mechanisms of PRDX-regulatory control.

KW - antioxidant

KW - cytokines

KW - exercise

KW - inflammation

KW - Redox status

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JO - Free Radical Research

JF - Free Radical Research

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