Monitoring of transcriptional dynamics of HIF and NFκB activities

Miguel A.S. Cavadas, Alex Cheong

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Genetic experiments over the last few decades have identified many regulatory proteins critical for DNA transcription. The dynamics of their transcriptional activities shape the differential expression of the genes they control. Here we describe a simple method, based on the secreted luciferase, to measure the activities of two transcription factors NF?B and HIF. This technique can effectively monitor dynamics of transcriptional events in a population of cells and be up-scaled for high-throughput screening and promoter analysis, making it ideal for data-demanding applications such as mathematical modelling.
Original languageEnglish
Title of host publicationBioluminescent imaging
Subtitle of host publicationmethods and protocols
EditorsChristian E. Badr
PublisherHumana Press
Pages97-105
Number of pages9
ISBN (Electronic)978-1-62703-718-1
ISBN (Print)978-1-62703-717-4
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume1098
ISSN (Print)1064-3745

Keywords

  • NFκB
  • HIF
  • transcription
  • luciferase
  • mathematical modelling

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  • Cite this

    Cavadas, M. A. S., & Cheong, A. (2014). Monitoring of transcriptional dynamics of HIF and NFκB activities. In C. E. Badr (Ed.), Bioluminescent imaging: methods and protocols (pp. 97-105). (Methods in molecular biology; Vol. 1098). Humana Press. https://doi.org/10.1007/978-1-62703-718-1_8