Genetic experiments over the last few decades have identified many regulatory proteins critical for DNA transcription. The dynamics of their transcriptional activities shape the differential expression of the genes they control. Here we describe a simple method, based on the secreted luciferase, to measure the activities of two transcription factors NF?B and HIF. This technique can effectively monitor dynamics of transcriptional events in a population of cells and be up-scaled for high-throughput screening and promoter analysis, making it ideal for data-demanding applications such as mathematical modelling.
|Title of host publication||Bioluminescent imaging|
|Subtitle of host publication||methods and protocols|
|Editors||Christian E. Badr|
|Number of pages||9|
|Publication status||Published - 2014|
|Name||Methods in molecular biology|
- mathematical modelling