This paper describes the experimental monitoring of pancreatic oxidative metabolism in laboratory mice that combines the methods of fluorescence and diffuse reflectance spectroscopy and laser speckle contrast imaging with a pancreatic ischemia model. The combined measurements show a close dependence of tissue metabolism on blood and oxygen supply. We show that deactivation of complex I and complex II occurs in mouse pancreatic tissue during prolonged hypoxia. We conclude that complex I can potentially undergo more intensive deactivation when oxygen is lacking than complex II. We have demonstrated that the methods described can be applied in minimally invasive surgery of the pancreas to assess its viability.
- Diffuse reflectance
- Laser speckle contrast imaging
- Minimally invasive surgery