Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure

Adam J. Watkins, Duncan Platt, Tom Papenbrock, Adrian Wilkins, Judith J. Eckert, Wing Yee Kwong, Clive Osmond, Mark Hanson, Tom P. Fleming

Research output: Contribution to journalArticle

Abstract

A key factor in the use of assisted reproductive technologies (ART) for diverse species is the safety of procedures for long-term health. By using a mouse model, we have investigated the effect of in vitro culture and embryo transfer (ET) of superovulated embryos on postnatal growth and physiological activity compared with that of embryos developing in vivo. Embryo culture from two-cell to blastocyst stages in T6 medium either with or without a protein source reduced blastocyst trophectoderm and inner cell mass cell number compared with that of embryos developing in vivo. Embryo culture and ET had minimal effects on postnatal growth when compared with in vivo development with an equivalent litter size. However, embryo culture, and to a lesser extent ET, led to an enhanced systolic blood pressure at 21 weeks compared with in vivo development independent of litter size, maternal origin, or body weight. Moreover, activity of enzymatic regulators of cardiovascular and metabolic physiology, namely, serum angiotensin-converting enzyme and the gluconeogenesis controller, hepatic phosphoeno/pyruvate carboxykinase, were significantly elevated in response to embryo culture and/or ET in female offspring at 27 weeks, independent of maternal factors and postnatal growth. These animal data indicate that postnatal physiological criteria important in cardiovascular and metabolic health may be more sensitive to routine ART procedures than growth.
Original languageEnglish
Pages (from-to)5449-5454
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume104
Issue number13
DOIs
Publication statusPublished - 27 Mar 2007

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embryo culture
systolic blood pressure
embryo transfer
phenotype
assisted reproductive technologies
embryo (animal)
mice
blastocyst
litter size
gluconeogenesis
peptidyl-dipeptidase A
controllers
cells
in vitro culture
protein sources
physiology
animal models
liver
body weight
animals

Keywords

  • assisted reproductive technologies
  • in vitro culture

Cite this

Watkins, A. J., Platt, D., Papenbrock, T., Wilkins, A., Eckert, J. J., Kwong, W. Y., ... Fleming, T. P. (2007). Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure. Proceedings of the National Academy of Sciences, 104(13), 5449-5454. https://doi.org/10.1073/pnas.0610317104
Watkins, Adam J. ; Platt, Duncan ; Papenbrock, Tom ; Wilkins, Adrian ; Eckert, Judith J. ; Kwong, Wing Yee ; Osmond, Clive ; Hanson, Mark ; Fleming, Tom P. / Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure. In: Proceedings of the National Academy of Sciences. 2007 ; Vol. 104, No. 13. pp. 5449-5454.
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Watkins, AJ, Platt, D, Papenbrock, T, Wilkins, A, Eckert, JJ, Kwong, WY, Osmond, C, Hanson, M & Fleming, TP 2007, 'Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure', Proceedings of the National Academy of Sciences, vol. 104, no. 13, pp. 5449-5454. https://doi.org/10.1073/pnas.0610317104

Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure. / Watkins, Adam J.; Platt, Duncan; Papenbrock, Tom; Wilkins, Adrian; Eckert, Judith J.; Kwong, Wing Yee; Osmond, Clive; Hanson, Mark; Fleming, Tom P.

In: Proceedings of the National Academy of Sciences, Vol. 104, No. 13, 27.03.2007, p. 5449-5454.

Research output: Contribution to journalArticle

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AU - Osmond, Clive

AU - Hanson, Mark

AU - Fleming, Tom P.

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