Muscle-derived precursor cells isolated on the basis of differential adhesion properties respond differently to capillary flow

Minal Patel, Hayley Mulhall, Khalid Al-Quatani, Mark Lewis, Ivan Wall*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Capillary shear stress can improve osteogenic differentiation in muscle-derived precursor cells (MDPCs). This has implications for large-scale bioprocessing of cell therapies where capillary transfer is needed. The recovery, viability, and osteogenic differentiation potential of two subsets of MDPCs, early-adherent pre-plate 1 (PP1) and late-adherent PP3 populations, have been examined: PP1 MDPCs produced a greater degree of osteogenic differentiation than PP3 MDPCs, quantified by Alizarin Red S staining intensity (P < 0.05). For both cell populations, capillary flow-induced significant increases in Alizarin Red S staining (P < 0.05). However, PP1 cells were more susceptible to capillary flow-induced damage than PP3 cells and this was dependent on duration of exposure. Overall, results indicate that different cell subsets, even from within a single tissue, can respond variably to capillary shear stress, necessitating its precise monitoring and control.

Original languageEnglish
Pages (from-to)1481-1486
Number of pages6
JournalBiotechnology Letters
Volume33
Issue number7
DOIs
Publication statusPublished - 1 Jul 2011

Bibliographical note

© Springer Science+Business Media B.V. 2011. The final publication is available at Springer via http://dx.doi.org/10.1007/s10529-011-0570-3

Keywords

  • Differential adhesion
  • Muscle-derived precursor cells
  • Osteogenic differentiation
  • Shear stress
  • Stem cells

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