TY - JOUR
T1 - New expanded bed adsorbents for the recovery of DNA
AU - Theodosiou, Eirini
AU - Olander, Morten A
AU - Søndergaard, Michael
AU - Thomas, Owen R.T.
PY - 2000
Y1 - 2000
N2 - A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.
AB - A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.
KW - anion exchange adsorbents
KW - DNA
KW - expanded bed chromatography
KW - gene therapy
UR - https://link.springer.com/article/10.1023%2FA%3A1026708603888
U2 - 10.1023/A:1026708603888
DO - 10.1023/A:1026708603888
M3 - Article
SN - 0141-5492
VL - 22
SP - 1929
EP - 1933
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 24
ER -