New expanded bed adsorbents for the recovery of DNA

Eirini Theodosiou, Morten A Olander, Michael Søndergaard, Owen R.T. Thomas

Research output: Contribution to journalArticle

Abstract

A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.
Original languageEnglish
Pages (from-to)1929-1933
JournalBiotechnology Letters
Volume22
Issue number24
DOIs
Publication statusPublished - 2000

Keywords

  • anion exchange adsorbents
  • DNA
  • expanded bed chromatography
  • gene therapy

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  • Cite this

    Theodosiou, E., Olander, M. A., Søndergaard, M., & Thomas, O. R. T. (2000). New expanded bed adsorbents for the recovery of DNA. Biotechnology Letters, 22(24), 1929-1933. https://doi.org/10.1023/A:1026708603888