New expanded bed adsorbents for the recovery of DNA

Eirini Theodosiou; Morten A Olander; Michael Søndergaard; Owen R.T.  Thomas

doi:10.1023/A:1026708603888

New expanded bed adsorbents for the recovery of DNA

Eirini Theodosiou
, Morten A Olander
, Michael Søndergaard
, Owen R.T. Thomas

College of Engineering and Physical Sciences

Upfront Chromatography
Danmarks Tekniske Universitet

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A 20–40 μm pellicular high density (∼3.7 g cm⁻³) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml⁻¹, c.f. ∼50 μg ml⁻¹ at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.

Original language	English
Pages (from-to)	1929-1933
Journal	Biotechnology Letters
Volume	22
Issue number	24
DOIs	https://doi.org/10.1023/A:1026708603888
Publication status	Published - 2000

Keywords

anion exchange adsorbents
DNA
expanded bed chromatography
gene therapy

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10.1023/A:1026708603888

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abstract = "A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10\% breakthrough), due to a combination of small particle and {\textquoteleft}fuzzy{\textquoteright} surface architecture created through the coupling of polyethylene imine chains.",

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AU - Olander, Morten A

AU - Søndergaard, Michael

AU - Thomas, Owen R.T.

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Y1 - 2000

N2 - A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.

AB - A 20–40 μm pellicular high density (∼3.7 g cm−3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml−1, c.f. ∼50 μg ml−1 at 10% breakthrough), due to a combination of small particle and ‘fuzzy’ surface architecture created through the coupling of polyethylene imine chains.

KW - anion exchange adsorbents

KW - DNA

KW - expanded bed chromatography

KW - gene therapy

UR - https://link.springer.com/article/10.1023%2FA%3A1026708603888

U2 - 10.1023/A:1026708603888

DO - 10.1023/A:1026708603888

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 24

ER -

New expanded bed adsorbents for the recovery of DNA

Abstract

Keywords

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