NKG2D ligands mediate immunosurveillance of senescent cells

Adi Sagiv, Dominick G.A. Burton, Zhana Moshayev, Ezra Vadai, Felix Wensveen, Shifra Ben-Dor, Ofra Golani, Bojan Polic, Valery Krizhanovsky

Research output: Contribution to journalArticle

Abstract

Cellular senescence is a stress response mechanism that limits tumorigenesis and tissue damage. Induction of cellular senescence commonly coincides with an immunogenic phenotype that promotes self-elimination by components of the immune system, thereby facilitating tumor suppression and limiting excess fibrosis during wound repair. The mechanisms by which senescent cells regulate their immune surveillance are not completely understood. Here we show that ligands of an activating Natural Killer (NK) cell receptor (NKG2D), MICA and ULBP2 are consistently up-regulated following induction of replicative senescence, oncogene-induced senescence and DNA damage - induced senescence. MICA and ULBP2 proteins are necessary for efficient NK-mediated cytotoxicity towards senescent fibroblasts. The mechanisms regulating the initial expression of NKG2D ligands in senescent cells are dependent on a DNA damage response, whilst continuous expression of these ligands is regulated by the ERK signaling pathway. In liver fibrosis, the accumulation of senescent activated stellate cells is increased in mice lacking NKG2D receptor leading to increased fibrosis. Overall, our results provide new insights into the mechanisms regulating the expression of immune ligands in senescent cells and reveal the importance of NKG2D receptor-ligand interaction in protecting against liver fibrosis.

LanguageEnglish
Pages328-344
Number of pages17
JournalAging
Volume8
Issue number2
DOIs
Publication statusPublished - 13 Feb 2016

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Immunologic Monitoring
Cell Aging
Ligands
NK Cell Lectin-Like Receptor Subfamily K
Liver Cirrhosis
DNA Damage
Fibrosis
Natural Killer Cell Receptors
MAP Kinase Signaling System
Oncogenes
Immune System
Carcinogenesis
Fibroblasts
Phenotype
Wounds and Injuries
Neoplasms
Proteins

Bibliographical note

Sagiv et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: Marie Curie RG grant; ERC EU’s FP7; and Israel Science Foundation

Keywords

  • DNA damage response
  • ERK
  • fFibrosis
  • natural killer cells
  • NKG2D
  • senescence

Cite this

Sagiv, A., Burton, D. G. A., Moshayev, Z., Vadai, E., Wensveen, F., Ben-Dor, S., ... Krizhanovsky, V. (2016). NKG2D ligands mediate immunosurveillance of senescent cells. Aging, 8(2), 328-344. https://doi.org/10.18632/aging.100897
Sagiv, Adi ; Burton, Dominick G.A. ; Moshayev, Zhana ; Vadai, Ezra ; Wensveen, Felix ; Ben-Dor, Shifra ; Golani, Ofra ; Polic, Bojan ; Krizhanovsky, Valery. / NKG2D ligands mediate immunosurveillance of senescent cells. In: Aging. 2016 ; Vol. 8, No. 2. pp. 328-344.
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Sagiv, A, Burton, DGA, Moshayev, Z, Vadai, E, Wensveen, F, Ben-Dor, S, Golani, O, Polic, B & Krizhanovsky, V 2016, 'NKG2D ligands mediate immunosurveillance of senescent cells' Aging, vol. 8, no. 2, pp. 328-344. https://doi.org/10.18632/aging.100897

NKG2D ligands mediate immunosurveillance of senescent cells. / Sagiv, Adi; Burton, Dominick G.A.; Moshayev, Zhana; Vadai, Ezra; Wensveen, Felix; Ben-Dor, Shifra; Golani, Ofra; Polic, Bojan; Krizhanovsky, Valery.

In: Aging, Vol. 8, No. 2, 13.02.2016, p. 328-344.

Research output: Contribution to journalArticle

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AU - Sagiv, Adi

AU - Burton, Dominick G.A.

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AU - Wensveen, Felix

AU - Ben-Dor, Shifra

AU - Golani, Ofra

AU - Polic, Bojan

AU - Krizhanovsky, Valery

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Sagiv A, Burton DGA, Moshayev Z, Vadai E, Wensveen F, Ben-Dor S et al. NKG2D ligands mediate immunosurveillance of senescent cells. Aging. 2016 Feb 13;8(2):328-344. https://doi.org/10.18632/aging.100897