TY - GEN
T1 - Non-Invasive Near-Infrared Optogenetics
T2 - Neurophotonics II 2024
AU - Galiakhmetova, Diana
AU - Dremin, Viktor
AU - Koviarov, Aleksandr
AU - Stoliarov, Dmitrii
AU - Ngum, Neville
AU - Parri, Rheinallt
AU - Gorodetsky, Andrei
AU - Maimaris, Marios
AU - Shcherbakova, Daria M.
AU - Baloban, Mikhail
AU - Verkhusha, Vladislav V.
AU - Sokolovsky, Sergei
AU - Rafailov, Edik
PY - 2024/6/20
Y1 - 2024/6/20
N2 - This research focuses on the development of a non-invasive/minimally invasive optogenetic technique. The study delves into how visible (VIS) and near-infrared (NIR) light interacts with ex vivo mouse head tissues, highlighting the advantages of the NIR-II biological window for deeper tissue penetration and reduced light absorption and scattering. Our computer simulations and experimental results demonstrated that over 12% of initial light irradiation passes through 1 mm tissue (skin and skull), reaching the brain cortex, potentially enabling minimally invasive neural activation. Moreover, this work reveals the nonlinear optical properties of genetically engineered truncated monomeric and dimeric bacterial phytochromes, demonstrating their photoconversion efficiency of up to 73% in the NIR-II range and potential for optogenetics. This discovery opens new avenues in advanced neurostimulation and biomedical research by enhancing tissue penetration and minimizing invasiveness.
AB - This research focuses on the development of a non-invasive/minimally invasive optogenetic technique. The study delves into how visible (VIS) and near-infrared (NIR) light interacts with ex vivo mouse head tissues, highlighting the advantages of the NIR-II biological window for deeper tissue penetration and reduced light absorption and scattering. Our computer simulations and experimental results demonstrated that over 12% of initial light irradiation passes through 1 mm tissue (skin and skull), reaching the brain cortex, potentially enabling minimally invasive neural activation. Moreover, this work reveals the nonlinear optical properties of genetically engineered truncated monomeric and dimeric bacterial phytochromes, demonstrating their photoconversion efficiency of up to 73% in the NIR-II range and potential for optogenetics. This discovery opens new avenues in advanced neurostimulation and biomedical research by enhancing tissue penetration and minimizing invasiveness.
KW - light-tissue interaction
KW - monomeric phytochrome
KW - optogenetics
KW - penetration depth
KW - second near-infrared window
KW - two-photon absorption
KW - two-photon fluorescence
KW - ultrashort pulsed fiber laser
UR - http://www.scopus.com/inward/record.url?scp=85199020002&partnerID=8YFLogxK
UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/13007/3016681/Non-invasive-near-infrared-optogenetics--aspirational-dream-or-approaching/10.1117/12.3016681.full
U2 - 10.1117/12.3016681
DO - 10.1117/12.3016681
M3 - Conference publication
AN - SCOPUS:85199020002
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Neurophotonics II
A2 - Cizmar, Tomas
A2 - Fellin, Tommaso
Y2 - 8 April 2024 through 9 April 2024
ER -