Noninvasive control of the transport function of fluorescent coloured liposomal nanoparticles

O. Stelmashchuk, E. Zherebtsov, A. Zherebtsova, E. Kuznetsova, A. Vinokurov, A. Dunaev, A. Mamoshin, I. Snimshchikova, A. Borsukov, A. Bykov, Igor Meglinski

Research output: Contribution to journalArticlepeer-review

Abstract

The use of liposomal nanoparticles with an incorporated active substance is an innovative and promising approach to diagnostics and therapy. The application of liposomal nanoparticle-based drugs allows for targeted localized delivery, overcomes the natural barriers within the body effectively, and minimizes possible side effects. Liposomes are able to contain a variety of ingredients with practically no limitations to their chemical composition, chemical properties, or size of constituent molecules. This study evaluated the ability to control the passage of fluorescent dye-filled liposomes through the intestinal mucosal barrier after oral administration. For this purpose, the increase in transcutaneous registered fluorescence from tetrabromofluorescein dye was recorded and analysed. Fluorescence intensity was measured at the proximal end of the tail of an animal model after oral administration of the liposomes. Measurements were taken at the excitation wavelengths of 365 and 450 nm. The fluorescence intensity in the group treated with the fluorescent contrast agent encapsulated in liposomal particles increased 140% of the initial level, but in the group treated with pure contrast agent, the increase in detected fluorescence intensity did not exceed 110%. Mice that received empty liposomes as well as the control group did not demonstrate statistically significant changes in fluorescence intensity. A potential application of our results is an express laser optical method of monitoring the transport of orally administered liposomal particles. The results can be used to help create new optical tools for use in the development of new drugs and in high-throughput screening used during their testing.
Original languageEnglish
Article number065603
Number of pages6
JournalLaser Physics Letters
Volume14
Issue number6
DOIs
Publication statusPublished - 8 May 2017

Bibliographical note

This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/1612-202X/aa6ef5

Funding: H2020

Keywords

  • laser fluorescence spectroscopy
  • optical diagnostics
  • drug delivery
  • nanoparticles
  • liposomes

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