Novel repair action of vitamin C upon in vivo oxidative DNA damage

Marcus S. Cooke, Mark D. Evans, Ian D. Podmore, Karl E. Herbert, Nalini Mistry, Pratibha Mistry, Peter T. Hickenbotham, Amina Hussieni, Helen R. Griffiths, Joseph Lunec

Research output: Contribution to journalArticlepeer-review


There appears to be a paucity of data examining the effect of dietary antioxidants on levels of oxidative DNA damage in vivo, limiting evidence-based assessment of antioxidant efficacy, mechanisms and recommendation for optimal intake. We have examined levels of 8-oxo-2'-deoxyguanosine (8-oxodG) in mononuclear cell DNA, serum and urine from subjects undergoing supplementation with 500 mg/day vitamin C. Significant decreases in DNA levels of 8-oxodG were seen, correlating strongly with increases in plasma vitamin C concentration. Furthermore we established a timecourse for sequential, significant increases in serum and urinary 8-oxodG levels. These results illustrate, for the first time in humans, the kinetics of 8-oxodG removal and processing in vivo, suggesting a role for vitamin C in the regulation of DNA repair enzymes and thereby demonstrating a non-scavenging antioxidant effect.
Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalFEBS Letters
Issue number3
Publication statusPublished - 20 Nov 1998


  • antioxidant
  • vitamin C
  • antibody
  • 8-Oxo-2′-deoxyguanosine
  • DNA repair
  • DNA damage


Dive into the research topics of 'Novel repair action of vitamin C upon in vivo oxidative DNA damage'. Together they form a unique fingerprint.

Cite this