NT2 derived neuronal and astrocytic network signalling

Eric Hill, Maria Jiménez-González, Marta Tarczyluk, David A. Nagel, Mike D. Coleman, Rheinallt Parri

Research output: Contribution to journalArticle

Abstract

A major focus of stem cell research is the generation of neurons that may then be implanted to treat neurodegenerative diseases. However, a picture is emerging where astrocytes are partners to neurons in sustaining and modulating brain function. We therefore investigated the functional properties of NT2 derived astrocytes and neurons using electrophysiological and calcium imaging approaches. NT2 neurons (NT2Ns) expressed sodium dependent action potentials, as well as responses to depolarisation and the neurotransmitter glutamate. NT2Ns exhibited spontaneous and coordinated calcium elevations in clusters and in extended processes, indicating local and long distance signalling. Tetrodotoxin sensitive network activity could also be evoked by electrical stimulation. Similarly, NT2 astrocytes (NT2As) exhibited morphology and functional properties consistent with this glial cell type. NT2As responded to neuronal activity and to exogenously applied neurotransmitters with calcium elevations, and in contrast to neurons, also exhibited spontaneous rhythmic calcium oscillations. NT2As also generated propagating calcium waves that were gap junction and purinergic signalling dependent. Our results show that NT2 derived astrocytes exhibit appropriate functionality and that NT2N networks interact with NT2A networks in co-culture. These findings underline the utility of such cultures to investigate human brain cell type signalling under controlled conditions. Furthermore, since stem cell derived neuron function and survival is of great importance therapeutically, our findings suggest that the presence of complementary astrocytes may be valuable in supporting stem cell derived neuronal networks. Indeed, this also supports the intriguing possibility of selective therapeutic replacement of astrocytes in diseases where these cells are either lost or lose functionality.
LanguageEnglish
Article numbere36098
Number of pages10
JournalPLoS ONE
Volume7
Issue number5
DOIs
Publication statusPublished - 2 May 2012

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astrocytes
Astrocytes
Neurons
neurons
Calcium
calcium
Stem cells
stem cells
Calcium Signaling
neurotransmitters
functional properties
Neurotransmitter Agents
Brain
Stem Cells
Neurodegenerative diseases
brain
Stem Cell Research
tetrodotoxin
gap junctions
Gap Junctions

Bibliographical note

© 2012 Hill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Cite this

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NT2 derived neuronal and astrocytic network signalling. / Hill, Eric; Jiménez-González, Maria; Tarczyluk, Marta; Nagel, David A.; Coleman, Mike D.; Parri, Rheinallt.

In: PLoS ONE, Vol. 7, No. 5, e36098, 02.05.2012.

Research output: Contribution to journalArticle

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