Observation of arenavirus nucleoprotein heptamer assembly

Nicolas Papageorgiou; Afroditi Vaitsopoulou; Awa Diop; Thi Hong Van Nguyen; Bruno Canard; Karine Alvarez; François Ferron

doi:10.1002/2211-5463.13106

Observation of arenavirus nucleoprotein heptamer assembly

Nicolas Papageorgiou, Afroditi Vaitsopoulou, Awa Diop, Thi Hong Van Nguyen, Bruno Canard, Karine Alvarez, François Ferron

Research output: Contribution to journal › Article › peer-review

Abstract

Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single-stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self-assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full-length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.

Original language	English
Pages (from-to)	1076-1083
Number of pages	8
Journal	FEBS Open Bio
Volume	11
Issue number	4
Early online date	25 Feb 2021
DOIs	https://doi.org/10.1002/2211-5463.13106
Publication status	Published - Apr 2021

Bibliographical note

© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Funding: Agence Nationale de la Recherche. Grant Numbers: FRISBI - ANR-10-INSB-05-01, PANUVI - ANR-18-ASTR-0010-01
Fondation Infection Méditerranée

Keywords

General Biochemistry, Genetics and Molecular Biology

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10.1002/2211-5463.13106Licence: CC BY 3.0

Observation of arenavirus nucleoprotein heptamer assembly
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 7.26 MBLicence: CC BY 3.0

Cite this

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title = "Observation of arenavirus nucleoprotein heptamer assembly",

abstract = "Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single-stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self-assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full-length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.",

keywords = "General Biochemistry, Genetics and Molecular Biology",

author = "Nicolas Papageorgiou and Afroditi Vaitsopoulou and Awa Diop and Nguyen, {Thi Hong Van} and Bruno Canard and Karine Alvarez and Fran{\c c}ois Ferron",

note = "{\textcopyright} 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Funding: Agence Nationale de la Recherche. Grant Numbers: FRISBI - ANR-10-INSB-05-01, PANUVI - ANR-18-ASTR-0010-01 Fondation Infection M{\'e}diterran{\'e}e",

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AU - Vaitsopoulou, Afroditi

AU - Diop, Awa

AU - Nguyen, Thi Hong Van

AU - Canard, Bruno

AU - Alvarez, Karine

AU - Ferron, François

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Observation of arenavirus nucleoprotein heptamer assembly

Abstract

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