Observation of arenavirus nucleoprotein heptamer assembly

Nicolas Papageorgiou, Afroditi Vaitsopoulou, Awa Diop, Thi Hong Van Nguyen, Bruno Canard, Karine Alvarez, François Ferron

Research output: Contribution to journalArticlepeer-review


Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single-stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self-assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full-length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.
Original languageEnglish
Pages (from-to)1076-1083
Number of pages8
JournalFEBS Open Bio
Issue number4
Early online date25 Feb 2021
Publication statusPublished - Apr 2021

Bibliographical note

© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Funding: Agence Nationale de la Recherche. Grant Numbers: FRISBI - ANR-10-INSB-05-01, PANUVI - ANR-18-ASTR-0010-01
Fondation Infection Méditerranée


  • General Biochemistry, Genetics and Molecular Biology


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