Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Kisung Lee, Matti Kinnunen, Maria D. Khokhlova, Evgeny V. Lyubin, Alexander V. Priezzhev*, Igor Meglinski, Andrey A. Fedyanin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

Original languageEnglish
Article number035001
JournalJournal of Biomedical Optics
Issue number3
Publication statusPublished - 8 Mar 2016

Bibliographical note

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this
work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.21.3.035001]


  • aggregation
  • albumin
  • blood microcirculation
  • cells interaction
  • fibrinogen
  • optical tweezers
  • red blood cell


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