Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Kisung Lee; Matti Kinnunen; Maria D. Khokhlova; Evgeny V. Lyubin; Alexander V. Priezzhev; Igor Meglinski; Andrey A. Fedyanin

doi:10.1117/1.JBO.21.3.035001

Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Kisung Lee, Matti Kinnunen, Maria D. Khokhlova, Evgeny V. Lyubin, Alexander V. Priezzhev^*, Igor Meglinski, Andrey A. Fedyanin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

Original language	English
Article number	035001
Journal	Journal of Biomedical Optics
Volume	21
Issue number	3
DOIs	https://doi.org/10.1117/1.JBO.21.3.035001
Publication status	Published - 8 Mar 2016

Bibliographical note

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this
work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.21.3.035001]

Keywords

aggregation
albumin
blood microcirculation
cells interaction
fibrinogen
optical tweezers
red blood cell

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title = "Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions",

abstract = "Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.",

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AU - Kinnunen, Matti

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AU - Lyubin, Evgeny V.

AU - Priezzhev, Alexander V.

AU - Meglinski, Igor

AU - Fedyanin, Andrey A.

N1 - © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.21.3.035001]

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Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

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