A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.
|Title of host publication||Recombinant protein production in yeast|
|Subtitle of host publication||methods and protocols|
|Editors||Roslyn M. Bill|
|Number of pages||10|
|Publication status||Published - 25 Feb 2012|
|Name||Methods in molecular biology|
- mixed feeds