Optimising Pichia pastoris induction

Zharain Bawa; Richard A.J. Darby

doi:10.1007/978-1-61779-770-5_15

Optimising Pichia pastoris induction

Zharain Bawa^*
, Richard A.J. Darby

^*Corresponding author for this work

John Radcliffe Hospital
Oxford Brookes University

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

6 Citations (SciVal)

Abstract

A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.

Original language	English
Title of host publication	Recombinant protein production in yeast
Subtitle of host publication	methods and protocols
Editors	Roslyn M. Bill
Publisher	Humana Press
Pages	181-190
Number of pages	10
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5_15
Publication status	Published - 25 Feb 2012

Publication series

Name	Methods in molecular biology
Publisher	Humana Press
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Keywords

methanol
mixed feeds
optimisation
yeast

Access to Document

10.1007/978-1-61779-770-5_15

Cite this

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KW - mixed feeds

KW - optimisation

KW - yeast

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BT - Recombinant protein production in yeast

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ER -

Optimising Pichia pastoris induction

Abstract

Publication series

Keywords

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Other files and links

Fingerprint

Large-scale production of secreted proteins in Pichia pastoris

Optimising yeast as a host for recombinant protein production (review)

Preface

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