Optimising yeast as a host for recombinant protein production (review)

Nicklas Bonander, Roslyn M. Bill

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Having access to suitably stable, functional recombinant protein samples underpins diverse academic and industrial research efforts to understand the workings of the cell in health and disease. Synthesising a protein in recombinant host cells typically allows the isolation of the pure protein in quantities much higher than those found in the protein's native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to the native human source cells of many proteins of interest, while also being quick, easy, and cheap to grow and process. Even in these cells the production of some proteins can be plagued by low functional yields. We have identified molecular mechanisms and culture parameters underpinning high yields and have consolidated our findings to engineer improved yeast cell factories. In this chapter, we provide an overview of the opportunities available to improve yeast as a host system for recombinant protein production.
Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages1-9
Number of pages9
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Keywords

  • recombinant protein production
  • yeast
  • strain engineering
  • bioprocess control

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  • Research Output

    • 6 Chapter (peer-reviewed)
    • 1 Anthology
    • 1 Foreword/postscript

    Large-scale production of secreted proteins in Pichia pastoris

    Bora, N., 25 Feb 2012, Recombinant protein production in yeast. Bill, R. M. (ed.). Humana Press, p. 217-235 19 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Preface

    Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. vii 1 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingForeword/postscript

    Cite this

    Bonander, N., & Bill, R. M. (2012). Optimising yeast as a host for recombinant protein production (review). In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 1-9). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_1