Optimization of RAPD for fingerprinting Salmonella

A C Hilton, J G Banks, C W Penn

Research output: Contribution to journalArticle

Abstract

Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercially available PCR buffer optimization kit, a particularly promising primer was identified and RAPD conditions for a highly discriminatory and reproducible characterization of Salmonella isolates was achieved. In addition, a technique to obtain reproducible RAPD fingerprints of Salmonella isolates without the need to purify genomic DNA is described.
Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalLetters in Applied Microbiology
Volume24
Issue number4
DOIs
Publication statusPublished - Apr 1997

Keywords

  • Animals
  • Base Sequence
  • Buffers
  • DNA Fingerprinting
  • DNA Primers
  • DNA, Bacterial
  • Evaluation Studies as Topic
  • Food Microbiology
  • Random Amplified Polymorphic DNA Technique
  • Reproducibility of Results
  • Salmonella

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