Oxidation state-dependent protein-protein interactions in disulfide cascades

Despoina A.I. Mavridou, Emmanuel Saridakis, Paraskevi Kritsiligkou, Alan D. Goddard, Julie M. Stevens, Stuart J. Ferguson, Christina Redfield

Research output: Contribution to journalArticle

Abstract

Bacterial growth and pathogenicity depend on the correct formation of disulfide bonds, a process controlled by the Dsb system in the periplasm of Gram-negative bacteria. Proteins with a thioredoxin fold play a central role in this process. A general feature of thiol-disulfide exchange reactions is the need to avoid a long lived product complex between protein partners. We use a multidisciplinary approach, involving NMR, x-ray crystallography, surface plasmon resonance, mutagenesis, and in vivo experiments, to investigate the interaction between the two soluble domains of the transmembrane reductant conductor DsbD. Our results show oxidation state-dependent affinities between these two domains. These observations have implications for the interactions of the ubiquitous thioredoxin-like proteins with their substrates, provide insight into the key role played by a unique redox partner with an immunoglobulin fold, and are of general importance for oxidative protein-folding pathways in all organisms.

LanguageEnglish
Pages24943-24956
Number of pages14
JournalJournal of Biological Chemistry
Volume286
Issue number28
Early online date3 May 2011
DOIs
Publication statusPublished - 15 Jul 2011

Fingerprint

Disulfides
Thioredoxins
Oxidation
Protein folding
Periplasm
Mutagenesis
Crystallography
Proteins
Surface Plasmon Resonance
Protein Folding
Reducing Agents
Surface plasmon resonance
Gram-Negative Bacteria
Sulfhydryl Compounds
Oxidation-Reduction
Virulence
Immunoglobulins
Bacteria
Nuclear magnetic resonance
X-Rays

Keywords

  • disulfide
  • NMR
  • oxidation-reduction
  • protein-protein interactions
  • thioredoxin
  • thiol-disulfide exchange
  • oxidoreductases
  • DsbD
  • X-ray crystallography

Cite this

Mavridou, D. A. I., Saridakis, E., Kritsiligkou, P., Goddard, A. D., Stevens, J. M., Ferguson, S. J., & Redfield, C. (2011). Oxidation state-dependent protein-protein interactions in disulfide cascades. Journal of Biological Chemistry, 286(28), 24943-24956. https://doi.org/10.1074/jbc.M111.236141
Mavridou, Despoina A.I. ; Saridakis, Emmanuel ; Kritsiligkou, Paraskevi ; Goddard, Alan D. ; Stevens, Julie M. ; Ferguson, Stuart J. ; Redfield, Christina. / Oxidation state-dependent protein-protein interactions in disulfide cascades. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 28. pp. 24943-24956.
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Mavridou, DAI, Saridakis, E, Kritsiligkou, P, Goddard, AD, Stevens, JM, Ferguson, SJ & Redfield, C 2011, 'Oxidation state-dependent protein-protein interactions in disulfide cascades' Journal of Biological Chemistry, vol. 286, no. 28, pp. 24943-24956. https://doi.org/10.1074/jbc.M111.236141

Oxidation state-dependent protein-protein interactions in disulfide cascades. / Mavridou, Despoina A.I.; Saridakis, Emmanuel; Kritsiligkou, Paraskevi; Goddard, Alan D.; Stevens, Julie M.; Ferguson, Stuart J.; Redfield, Christina.

In: Journal of Biological Chemistry, Vol. 286, No. 28, 15.07.2011, p. 24943-24956.

Research output: Contribution to journalArticle

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Mavridou DAI, Saridakis E, Kritsiligkou P, Goddard AD, Stevens JM, Ferguson SJ et al. Oxidation state-dependent protein-protein interactions in disulfide cascades. Journal of Biological Chemistry. 2011 Jul 15;286(28):24943-24956. https://doi.org/10.1074/jbc.M111.236141