Oxygen radical induced alterations in polyclonal IgG

Helen R. Griffiths, Joseph Lunec, C.A. Gee, R.L. Willson

Research output: Contribution to journalArticle

Abstract

In the sera and synovial fluid of patients with rheumatoid arthritis, part of the IgG fraction is found in an aggregated and fluorescent form. Oxygen-free radicals have been implicated in this denaturation, although the precise radical species responsible is unknown. In this work, oxygen-free radicals generated radiolytically were allowed to attack polyclonal IgG in solution. OH radicals induced aggregation of the monomer and a new fluorescence appeared in the visible region (Ex 360 nm, Em 454 nm). The superoxide radical anion was found to be inert in both these respects, whilst peroxy radicals induced autofluorescence without concomitant aggregation. The results suggest that OH.and/or peroxy radical attack may be an in vivo mechanism for IgG denaturation.
Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalFEBS Letters
Volume230
Issue number1-2
DOIs
Publication statusPublished - 28 Mar 1988

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Reactive Oxygen Species
Denaturation
Immunoglobulin G
Superoxides
Free Radicals
Agglomeration
Oxygen
Synovial Fluid
Anions
Rheumatoid Arthritis
Monomers
Fluorescence
Fluids
Serum

Keywords

  • oxygen-free radical
  • fluorescence
  • IgG

Cite this

Griffiths, H. R., Lunec, J., Gee, C. A., & Willson, R. L. (1988). Oxygen radical induced alterations in polyclonal IgG. FEBS Letters, 230(1-2), 155-158. https://doi.org/10.1016/0014-5793(88)80661-6
Griffiths, Helen R. ; Lunec, Joseph ; Gee, C.A. ; Willson, R.L. / Oxygen radical induced alterations in polyclonal IgG. In: FEBS Letters. 1988 ; Vol. 230, No. 1-2. pp. 155-158.
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Griffiths, HR, Lunec, J, Gee, CA & Willson, RL 1988, 'Oxygen radical induced alterations in polyclonal IgG', FEBS Letters, vol. 230, no. 1-2, pp. 155-158. https://doi.org/10.1016/0014-5793(88)80661-6

Oxygen radical induced alterations in polyclonal IgG. / Griffiths, Helen R.; Lunec, Joseph; Gee, C.A.; Willson, R.L.

In: FEBS Letters, Vol. 230, No. 1-2, 28.03.1988, p. 155-158.

Research output: Contribution to journalArticle

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Griffiths HR, Lunec J, Gee CA, Willson RL. Oxygen radical induced alterations in polyclonal IgG. FEBS Letters. 1988 Mar 28;230(1-2):155-158. https://doi.org/10.1016/0014-5793(88)80661-6