Particulate material for purification of bio-macromolecules

Morten A. Olander (Inventor), Allan O.F. Lihme (Inventor), Timothy J. Hobley (Inventor), Marcos Simon (Inventor), Irene Theodossiou (Inventor), Owen R.T. Thomas (Inventor)

Research output: Patent

Abstract

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
Original languageEnglish
Patent numberUS6783962 B1
Priority date26/03/99
Publication statusPublished - 31 Aug 2004

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Macromolecules
Purification
Polyethyleneimine
Steel
DNA
Viral DNA
Patents and inventions
Chromatography
Titanium
RNA
Viruses
Sepharose
Fluidized beds
Polysaccharides
Bacteria
Plasmids
Ligands
Molecules

Cite this

Olander, M. A., Lihme, A. O. F., Hobley, T. J., Simon, M., Theodossiou, I., & Thomas, O. R. T. (2004). Particulate material for purification of bio-macromolecules. (Patent No. US6783962 B1).
Olander, Morten A. (Inventor) ; Lihme, Allan O.F. (Inventor) ; Hobley, Timothy J. (Inventor) ; Simon, Marcos (Inventor) ; Theodossiou, Irene (Inventor) ; Thomas, Owen R.T. (Inventor). / Particulate material for purification of bio-macromolecules. Patent No.: US6783962 B1.
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abstract = "The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.",
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Olander, MA, Lihme, AOF, Hobley, TJ, Simon, M, Theodossiou, I & Thomas, ORT 2004, Particulate material for purification of bio-macromolecules, Patent No. US6783962 B1.

Particulate material for purification of bio-macromolecules. / Olander, Morten A. (Inventor); Lihme, Allan O.F. (Inventor); Hobley, Timothy J. (Inventor); Simon, Marcos (Inventor); Theodossiou, Irene (Inventor); Thomas, Owen R.T. (Inventor).

Patent No.: US6783962 B1.

Research output: Patent

TY - PAT

T1 - Particulate material for purification of bio-macromolecules

AU - Olander, Morten A.

AU - Lihme, Allan O.F.

AU - Hobley, Timothy J.

AU - Simon, Marcos

AU - Theodossiou, Irene

AU - Thomas, Owen R.T.

PY - 2004/8/31

Y1 - 2004/8/31

N2 - The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

AB - The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

M3 - Patent

M1 - US6783962 B1

ER -

Olander MA, Lihme AOF, Hobley TJ, Simon M, Theodossiou I, Thomas ORT, inventors. Particulate material for purification of bio-macromolecules. US6783962 B1. 2004 Aug 31.