Particulate material for purification of bio-macromolecules

Morten A. Olander; Allan O.F. Lihme; Timothy J. Hobley; Marcos Simon; Irene Theodossiou; Owen R.T. Thomas

Particulate material for purification of bio-macromolecules

Morten A. Olander (Inventor), Allan O.F. Lihme (Inventor), Timothy J. Hobley (Inventor), Marcos Simon (Inventor), Irene Theodossiou (Inventor), Owen R.T. Thomas (Inventor)

College of Engineering and Physical Sciences

Research output: Patent

Abstract

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

Original language	English
Patent number	US6783962 B1
Priority date	26/03/99
Publication status	Published - 31 Aug 2004

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title = "Particulate material for purification of bio-macromolecules",

abstract = "The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.",

author = "Olander, {Morten A.} and Lihme, {Allan O.F.} and Hobley, {Timothy J.} and Marcos Simon and Irene Theodossiou and Thomas, {Owen R.T.}",

year = "2004",

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day = "31",

language = "English",

type = "Patent",

note = "US6783962 B1",

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TY - PAT

T1 - Particulate material for purification of bio-macromolecules

AU - Olander, Morten A.

AU - Lihme, Allan O.F.

AU - Hobley, Timothy J.

AU - Simon, Marcos

AU - Theodossiou, Irene

AU - Thomas, Owen R.T.

PY - 2004/8/31

Y1 - 2004/8/31

N2 - The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

AB - The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

M3 - Patent

M1 - US6783962 B1

ER -

Particulate material for purification of bio-macromolecules

Abstract

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