Performance and flow dynamics studies of polymeric optofluidic sers sensors

S. Uusitalo, J. Hiltunen, P. Karioja, S. Siitonen, V. Kontturi, R. Myllylä, M. Kinnunen, I. Meglinski

Research output: Contribution to journalArticle

Abstract

We present a polymer-based optofluidic surface enhanced Raman scattering chip for biomolecule detection, serving as a disposable sensorchoice with cost-effective production. The SERS substrate is fabricated by using industrial roll-to-roll UV-nanoimprinting equipment andintegrated with adhesive-based polymeric microfluidics. The functioning of the SERS detection on-chip is confirmed and the effect of thepolymer lid on the obtainable Raman spectra is analysed. Rhodamine 6G is used as a model analyte to demonstrate continuous flowmeasurements on a planar SERS substrate in a microchannel. The relation between the temporal response of the sensors and sample flowdynamics is studied with varied flow velocities, using SERS and fluorescence detection. The response time of the surface-dependent SERSsignal is longer than the response time of the fluorescence signal of the bulk flow. This observation revealed the effect of convection on thetemporal SERS responses at 25 μl/min to 1000 μl/min flow velocities. The diffusion of analyte molecules from the bulk concentration intothe sensing surface induces about a 40-second lag time in the SERS detection. This lag time, and its rising trend with slower flow velocities, has to be taken into account in future trials of the optofluidic SERS sensor, with active analyte binding on the sensing surface.

Original languageEnglish
JournalJournal of the European optical society
Volume10
DOIs
Publication statusPublished - 15 Sep 2015

Fingerprint

sensors
flow velocity
time lag
chips
Raman spectra
fluorescence
rhodamine
microchannels
adhesives
convection
costs
trends
polymers
molecules

Bibliographical note

The journal is fully open access. This means that anybody is free to read papers published by this journal without the need to register with the journal. On top of the open access, the authors of a published paper have the full copyright of their publication.

Keywords

  • Biosensor
  • Continuous
  • Flow
  • Optofluidic
  • Polymer
  • SERS

Cite this

Uusitalo, S. ; Hiltunen, J. ; Karioja, P. ; Siitonen, S. ; Kontturi, V. ; Myllylä, R. ; Kinnunen, M. ; Meglinski, I. / Performance and flow dynamics studies of polymeric optofluidic sers sensors. In: Journal of the European optical society. 2015 ; Vol. 10.
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Performance and flow dynamics studies of polymeric optofluidic sers sensors. / Uusitalo, S.; Hiltunen, J.; Karioja, P.; Siitonen, S.; Kontturi, V.; Myllylä, R.; Kinnunen, M.; Meglinski, I.

In: Journal of the European optical society, Vol. 10, 15.09.2015.

Research output: Contribution to journalArticle

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AU - Uusitalo, S.

AU - Hiltunen, J.

AU - Karioja, P.

AU - Siitonen, S.

AU - Kontturi, V.

AU - Myllylä, R.

AU - Kinnunen, M.

AU - Meglinski, I.

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