Abstract
| Language | English |
|---|---|
| Article number | P37 |
| Pages | 39 |
| Number of pages | 1 |
| Journal | Diabetic Medicine |
| Volume | 30 |
| Issue number | S1 |
| DOIs | |
| Publication status | Published - Mar 2013 |
| Event | Diabetes UK professional conference 2013 - Manchester Central Convention Complex, Manchester, United Kingdom Duration: 13 Jan 2015 → 15 Mar 2015 |
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Bibliographical note
Special Issue: Abstacts of the Diabetes UK Professional Conference 2013, Manchester Central Convention Complex, Manchester, UK, 13-15 March 2013Cite this
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Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function. / Choudry, N.; Robinson, J.; Arif, M.; Rana, K.S.; Hill, E.J.; Turner, S.; Bailey, C.J.; Brown, J.E.
In: Diabetic Medicine, Vol. 30, No. S1, P37, 03.2013, p. 39.Research output: Contribution to journal › Meeting abstract
TY - JOUR
T1 - Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function
AU - Choudry, N.
AU - Robinson, J.
AU - Arif, M.
AU - Rana, K.S.
AU - Hill, E.J.
AU - Turner, S.
AU - Bailey, C.J.
AU - Brown, J.E.
N1 - Special Issue: Abstacts of the Diabetes UK Professional Conference 2013, Manchester Central Convention Complex, Manchester, UK, 13-15 March 2013
PY - 2013/3
Y1 - 2013/3
N2 - Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25% at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000% compared with control, p < 0.001) and 2h glucose depletion from media (15% increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.
AB - Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25% at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000% compared with control, p < 0.001) and 2h glucose depletion from media (15% increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.
UR - http://doi.wiley.com/10.1111/dme.12091_1
U2 - 10.1111/dme.12091_1
DO - 10.1111/dme.12091_1
M3 - Meeting abstract
VL - 30
SP - 39
JO - Diabetic Medicine
T2 - Diabetic Medicine
JF - Diabetic Medicine
SN - 0742-3071
IS - S1
M1 - P37
ER -