Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function

N. Choudry, J. Robinson, M. Arif, K.S. Rana, E.J. Hill, S. Turner, C.J. Bailey, J.E. Brown

Research output: Contribution to journalMeeting abstract

Abstract

Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25% at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000% compared with control, p < 0.001) and 2h glucose depletion from media (15% increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.
Original languageEnglish
Article numberP37
Pages (from-to)39
Number of pages1
JournalDiabetic Medicine
Volume30
Issue numberS1
DOIs
Publication statusPublished - Mar 2013
EventDiabetes UK professional conference 2013 - Manchester Central Convention Complex, Manchester, United Kingdom
Duration: 13 Jan 201515 Mar 2015

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Phytoestrogens
Estrogens
Cell Proliferation
Sarcopenia
Glucose
Estrogen Receptor alpha
Research
Estrogen Receptors
Muscle Cells
Estradiol
Skeletal Muscle
Cell Line
2,3-bis(3'-hydroxybenzyl)butane-1,4-diol
2,3-bis(3'-hydroxybenzyl)butyrolactone
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose
Therapeutics

Bibliographical note

Special Issue: Abstacts of the Diabetes UK Professional Conference 2013, Manchester Central Convention Complex, Manchester, UK, 13-15 March 2013

Cite this

Choudry, N. ; Robinson, J. ; Arif, M. ; Rana, K.S. ; Hill, E.J. ; Turner, S. ; Bailey, C.J. ; Brown, J.E. / Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function. In: Diabetic Medicine. 2013 ; Vol. 30, No. S1. pp. 39.
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abstract = "Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25{\%} at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000{\%} compared with control, p < 0.001) and 2h glucose depletion from media (15{\%} increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.",
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Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function. / Choudry, N.; Robinson, J.; Arif, M.; Rana, K.S.; Hill, E.J.; Turner, S.; Bailey, C.J.; Brown, J.E.

In: Diabetic Medicine, Vol. 30, No. S1, P37, 03.2013, p. 39.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Phytoestrogen-ind uced glucose uptake and cellular proliferation in L6 myotubesadipocyte function

AU - Choudry, N.

AU - Robinson, J.

AU - Arif, M.

AU - Rana, K.S.

AU - Hill, E.J.

AU - Turner, S.

AU - Bailey, C.J.

AU - Brown, J.E.

N1 - Special Issue: Abstacts of the Diabetes UK Professional Conference 2013, Manchester Central Convention Complex, Manchester, UK, 13-15 March 2013

PY - 2013/3

Y1 - 2013/3

N2 - Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25% at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000% compared with control, p < 0.001) and 2h glucose depletion from media (15% increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.

AB - Aims: Oestrogens are known to act on a number of tissues throughout the body via classical oestrogen receptors, alpha (ER-a) and beta (ER-beta). Previous research has shown that oestrogens can regulate skeletal muscle glucose uptake cellular proliferation. Thus, oestrogens and related molecules provide an interesting focus for research into possible therapies for the treatment of metabolic disorders and sarcopenia. Enterodiol and enterolactone are plant derived mammalian enterolignans which share a struc- tural similarity to the human oestrogen oestradiol. Methods: In the present study we incubated the differentiated rat skeletal muscle cell line L6 concentration ranges of both com- pounds in the presence/absence of oestrogen receptor antagonists and measured glucose uptake using the non-metabolised glucose analogue 2-NBDG. Cellular proliferation was also measured using a modified MTS assay. Results: Enterolactone was seen to cause a significant increase in cellular proliferation after 48h (a maximal 25% at 0.1nmol/l), in an ER-a dependent mechanism. Incubation with 10nmol/l and 100nmol/l enterodiol caused significant increases in 2-NBDG (5000% compared with control, p < 0.001) and 2h glucose depletion from media (15% increase compared with control, p < 0.05), also in an ER-a dependent way. These results suggest these dietary derived oestrogen-like molecules might be of potential use in targeting metabolic disorders or sarcopenia. Conclusion: We can report here that the phytoestrogen derived molecules enterodiol and enterolactone interact with ER-a in the myotubes to regulate glucose uptake and cellular proliferation respectively.

UR - http://doi.wiley.com/10.1111/dme.12091_1

U2 - 10.1111/dme.12091_1

DO - 10.1111/dme.12091_1

M3 - Meeting abstract

VL - 30

SP - 39

JO - Diabetic Medicine

JF - Diabetic Medicine

SN - 0742-3071

IS - S1

M1 - P37

ER -