Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL

Manasanan Namhongsa; Donraporn Daranarong; Robert Molloy; Sukunya Ross; Gareth M. Ross; Adisorn Tuantranont; Dheerawan Boonyawan; Jiraporn Tocharus; Sivanan Sivasinprasasn; Paul D. Topham; Brian J. Tighe; Winita Punyodom

doi:10.1016/j.eurpolymj.2023.112135

Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL

Manasanan Namhongsa, Donraporn Daranarong, Robert Molloy, Sukunya Ross, Gareth M. Ross, Adisorn Tuantranont, Dheerawan Boonyawan, Jiraporn Tocharus, Sivanan Sivasinprasasn, Paul D. Topham, Brian J. Tighe, Winita Punyodom^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, two-component, morphologically composite scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with a nitrogen-argon (N₂-Ar) plasma to enhance their surface properties. The 3D-printed component provided mechanical strength, while the electrospun fibrous component acted as a mimic to the extracellular matrix to improve cell-substrate interactions. Two biodegradable polyesters, poly(L-lactide-co-ε-caprolactone) (PLCL) and poly(L-lactide-co-glycolide) (PLGA), were used to create the scaffolds. The resulting 3D/E/N₂-Ar scaffolds were characterized in terms of surface properties (morphology, chemical compositions, wettability, roughness, crystallinity), degradation, mechanical properties, and cell cytotoxicity, cell attachment and proliferation, LDH release and cell apoptosis. Results showed that the plasma treatment significantly increased the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided sufficient mechanical support, while the electrospun fiber component promoted cell attachment and proliferation. Following plasma treatment, the water contact angle of the scaffolds was greatly reduced from 124.0 ± 1.8° (PLCL) and 119.6 ± 1.4° (PLGA), to 0° and persisted even after 168 days. Human Schwann cells (SCs) showed excellent viability on both 3D/E/N₂-Ar and 3D/E scaffolds were in excess of 95%. Cells cultivated on the 3D/E/N₂-Ar scaffolds, with higher surface roughness, displayed significant increase in attachment and proliferation and a higher presence of healthy cells when compared with untreated 3D/E scaffolds. Both PLCL and PLGA scaffolds showed potential for use in biomedical applications. Although PLGA performed slightly better in terms of cell behavior, PLCL exhibited a slower degradation rate and higher tensile strain. These results demonstrate the potential of these designed scaffolds to support cell regeneration in clinically relevant devices such as nerve guide conduits and nerve protectant wraps.

Original language	English
Article number	112135
Number of pages	13
Journal	European Polymer Journal
Volume	194
Early online date	8 May 2023
DOIs	https://doi.org/10.1016/j.eurpolymj.2023.112135
Publication status	Published - 24 Jul 2023

Bibliographical note

Funding Information:
This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program (PHD/0026/2559) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL).

Funding Information:
This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program ( PHD/0026/2559 ) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL).

Publisher Copyright:
Copyright © 2023 Published by Elsevier Ltd.

Keywords

3D printing
Electrospinning
Plasma surface modification
Poly(L-lactide-co-glycolide)
Poly(L-lactide-co-ε-caprolactone)

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10.1016/j.eurpolymj.2023.112135

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Namhongsa, M., Daranarong, D., Molloy, R., Ross, S., Ross, G. M., Tuantranont, A., Boonyawan, D., Tocharus, J., Sivasinprasasn, S., Topham, P. D., Tighe, B. J., & Punyodom, W. (2023). Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL. European Polymer Journal, 194, Article 112135. https://doi.org/10.1016/j.eurpolymj.2023.112135

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title = "Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL",

abstract = "In this study, two-component, morphologically composite scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with a nitrogen-argon (N2-Ar) plasma to enhance their surface properties. The 3D-printed component provided mechanical strength, while the electrospun fibrous component acted as a mimic to the extracellular matrix to improve cell-substrate interactions. Two biodegradable polyesters, poly(L-lactide-co-ε-caprolactone) (PLCL) and poly(L-lactide-co-glycolide) (PLGA), were used to create the scaffolds. The resulting 3D/E/N2-Ar scaffolds were characterized in terms of surface properties (morphology, chemical compositions, wettability, roughness, crystallinity), degradation, mechanical properties, and cell cytotoxicity, cell attachment and proliferation, LDH release and cell apoptosis. Results showed that the plasma treatment significantly increased the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided sufficient mechanical support, while the electrospun fiber component promoted cell attachment and proliferation. Following plasma treatment, the water contact angle of the scaffolds was greatly reduced from 124.0 ± 1.8° (PLCL) and 119.6 ± 1.4° (PLGA), to 0° and persisted even after 168 days. Human Schwann cells (SCs) showed excellent viability on both 3D/E/N2-Ar and 3D/E scaffolds were in excess of 95%. Cells cultivated on the 3D/E/N2-Ar scaffolds, with higher surface roughness, displayed significant increase in attachment and proliferation and a higher presence of healthy cells when compared with untreated 3D/E scaffolds. Both PLCL and PLGA scaffolds showed potential for use in biomedical applications. Although PLGA performed slightly better in terms of cell behavior, PLCL exhibited a slower degradation rate and higher tensile strain. These results demonstrate the potential of these designed scaffolds to support cell regeneration in clinically relevant devices such as nerve guide conduits and nerve protectant wraps.",

keywords = "3D printing, Electrospinning, Plasma surface modification, Poly(L-lactide-co-glycolide), Poly(L-lactide-co-ε-caprolactone)",

author = "Manasanan Namhongsa and Donraporn Daranarong and Robert Molloy and Sukunya Ross and Ross, {Gareth M.} and Adisorn Tuantranont and Dheerawan Boonyawan and Jiraporn Tocharus and Sivanan Sivasinprasasn and Topham, {Paul D.} and Tighe, {Brian J.} and Winita Punyodom",

note = "Funding Information: This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program (PHD/0026/2559) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL). Funding Information: This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program ( PHD/0026/2559 ) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL). Publisher Copyright: Copyright {\textcopyright} 2023 Published by Elsevier Ltd.",

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doi = "10.1016/j.eurpolymj.2023.112135",

language = "English",

volume = "194",

journal = "European Polymer Journal",

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Namhongsa, M, Daranarong, D, Molloy, R, Ross, S, Ross, GM, Tuantranont, A, Boonyawan, D, Tocharus, J, Sivasinprasasn, S, Topham, PD , Tighe, BJ & Punyodom, W 2023, 'Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL', European Polymer Journal, vol. 194, 112135. https://doi.org/10.1016/j.eurpolymj.2023.112135

TY - JOUR

T1 - Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL

AU - Namhongsa, Manasanan

AU - Daranarong, Donraporn

AU - Molloy, Robert

AU - Ross, Sukunya

AU - Ross, Gareth M.

AU - Tuantranont, Adisorn

AU - Boonyawan, Dheerawan

AU - Tocharus, Jiraporn

AU - Sivasinprasasn, Sivanan

AU - Topham, Paul D.

AU - Tighe, Brian J.

AU - Punyodom, Winita

N1 - Funding Information: This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program (PHD/0026/2559) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL). Funding Information: This work was supported by a scholarship under The Royal Golden Jubilee for a Ph.D. Program ( PHD/0026/2559 ) and National Research University Project (NRU). The authors would like to thank the Department of Chemistry, Chiang Mai University, for providing the research facilities and the printed electronic for fabrication by the National Electronics and Computer Technology Center (NECTEC), NSTDA for financial support. This project was also partially funded from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 871650 (MEDIPOL). Publisher Copyright: Copyright © 2023 Published by Elsevier Ltd.

PY - 2023/7/24

Y1 - 2023/7/24

N2 - In this study, two-component, morphologically composite scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with a nitrogen-argon (N2-Ar) plasma to enhance their surface properties. The 3D-printed component provided mechanical strength, while the electrospun fibrous component acted as a mimic to the extracellular matrix to improve cell-substrate interactions. Two biodegradable polyesters, poly(L-lactide-co-ε-caprolactone) (PLCL) and poly(L-lactide-co-glycolide) (PLGA), were used to create the scaffolds. The resulting 3D/E/N2-Ar scaffolds were characterized in terms of surface properties (morphology, chemical compositions, wettability, roughness, crystallinity), degradation, mechanical properties, and cell cytotoxicity, cell attachment and proliferation, LDH release and cell apoptosis. Results showed that the plasma treatment significantly increased the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided sufficient mechanical support, while the electrospun fiber component promoted cell attachment and proliferation. Following plasma treatment, the water contact angle of the scaffolds was greatly reduced from 124.0 ± 1.8° (PLCL) and 119.6 ± 1.4° (PLGA), to 0° and persisted even after 168 days. Human Schwann cells (SCs) showed excellent viability on both 3D/E/N2-Ar and 3D/E scaffolds were in excess of 95%. Cells cultivated on the 3D/E/N2-Ar scaffolds, with higher surface roughness, displayed significant increase in attachment and proliferation and a higher presence of healthy cells when compared with untreated 3D/E scaffolds. Both PLCL and PLGA scaffolds showed potential for use in biomedical applications. Although PLGA performed slightly better in terms of cell behavior, PLCL exhibited a slower degradation rate and higher tensile strain. These results demonstrate the potential of these designed scaffolds to support cell regeneration in clinically relevant devices such as nerve guide conduits and nerve protectant wraps.

AB - In this study, two-component, morphologically composite scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with a nitrogen-argon (N2-Ar) plasma to enhance their surface properties. The 3D-printed component provided mechanical strength, while the electrospun fibrous component acted as a mimic to the extracellular matrix to improve cell-substrate interactions. Two biodegradable polyesters, poly(L-lactide-co-ε-caprolactone) (PLCL) and poly(L-lactide-co-glycolide) (PLGA), were used to create the scaffolds. The resulting 3D/E/N2-Ar scaffolds were characterized in terms of surface properties (morphology, chemical compositions, wettability, roughness, crystallinity), degradation, mechanical properties, and cell cytotoxicity, cell attachment and proliferation, LDH release and cell apoptosis. Results showed that the plasma treatment significantly increased the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided sufficient mechanical support, while the electrospun fiber component promoted cell attachment and proliferation. Following plasma treatment, the water contact angle of the scaffolds was greatly reduced from 124.0 ± 1.8° (PLCL) and 119.6 ± 1.4° (PLGA), to 0° and persisted even after 168 days. Human Schwann cells (SCs) showed excellent viability on both 3D/E/N2-Ar and 3D/E scaffolds were in excess of 95%. Cells cultivated on the 3D/E/N2-Ar scaffolds, with higher surface roughness, displayed significant increase in attachment and proliferation and a higher presence of healthy cells when compared with untreated 3D/E scaffolds. Both PLCL and PLGA scaffolds showed potential for use in biomedical applications. Although PLGA performed slightly better in terms of cell behavior, PLCL exhibited a slower degradation rate and higher tensile strain. These results demonstrate the potential of these designed scaffolds to support cell regeneration in clinically relevant devices such as nerve guide conduits and nerve protectant wraps.

KW - 3D printing

KW - Electrospinning

KW - Plasma surface modification

KW - Poly(L-lactide-co-glycolide)

KW - Poly(L-lactide-co-ε-caprolactone)

UR - https://www.sciencedirect.com/science/article/pii/S001430572300318X

U2 - 10.1016/j.eurpolymj.2023.112135

DO - 10.1016/j.eurpolymj.2023.112135

M3 - Article

AN - SCOPUS:85159582212

SN - 0014-3057

VL - 194

JO - European Polymer Journal

JF - European Polymer Journal

M1 - 112135

ER -

Plasma surface modification of two-component composite scaffolds consisting of 3D-printed and electrospun fiber components from biodegradable PLGA and PLCL

Abstract

Bibliographical note

Keywords

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