PLGA microspheres for the delivery of a novel subunit TB vaccine

Daniel J. Kirby, Ida Rosenkrands, Else M. Agger, Peter Andersen, Allan G.A. Coombes, Yvonne Perrie

Research output: Contribution to journalArticle

Abstract

Biodegradable poly(dl-lactide-co-glycolide) microspheres were prepared using a modified double emulsion solvent evaporation method for the delivery of the subunit tuberculosis vaccine (Ag85B-ESAT-6), a fusion protein of the immunodominant antigens 6-kDa early secretory antigenic target (ESAT-6) and antigen 85B (Ag85B). Addition of the cationic lipid dimethyl dioctadecylammonium bromide (DDA) and the immunostimulatory trehalose 6,6'-dibehenate (TDB), either separately or in combination, was investigated for the effect on particle size and distribution, antigen entrapment efficiency, in vitro release profiles and in vivo performance. Optimised formulation parameters yielded microspheres within the desired sub-10 mu m range (1.50 +/- 0.13 mu m), whilst exhibiting a high antigen entrapment efficiency (95 +/- 1.2%) and prolonged release profiles. Although the microsphere formulations induced a cell-mediated immune response and raised specific antibodies after immunisation, this was inferior to the levels achieved with liposomes composed of the same adjuvants (DDA-TDB), demonstrating that liposomes are more effective vaccine delivery systems compared with microspheres.
LanguageEnglish
Pages282-293
Number of pages12
JournalJournal of Drug Targeting
Volume16
Issue number4
DOIs
Publication statusPublished - May 2008

Fingerprint

Subunit Vaccines
Microspheres
Antigens
Bromides
Liposomes
Tuberculosis Vaccines
Polyglactin 910
Immunodominant Epitopes
Emulsions
Particle Size
Immunization
Vaccines
Lipids
polylactic acid-polyglycolic acid copolymer
Antibodies
Proteins
trehalose 6,6'-dibehenate

Keywords

  • microspheres
  • PLGA
  • adjuvants
  • subunit vaccines
  • tuberculosis

Cite this

Kirby, D. J., Rosenkrands, I., Agger, E. M., Andersen, P., Coombes, A. G. A., & Perrie, Y. (2008). PLGA microspheres for the delivery of a novel subunit TB vaccine. Journal of Drug Targeting, 16(4), 282-293. https://doi.org/10.1080/10611860801900462
Kirby, Daniel J. ; Rosenkrands, Ida ; Agger, Else M. ; Andersen, Peter ; Coombes, Allan G.A. ; Perrie, Yvonne. / PLGA microspheres for the delivery of a novel subunit TB vaccine. In: Journal of Drug Targeting. 2008 ; Vol. 16, No. 4. pp. 282-293.
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Kirby, DJ, Rosenkrands, I, Agger, EM, Andersen, P, Coombes, AGA & Perrie, Y 2008, 'PLGA microspheres for the delivery of a novel subunit TB vaccine' Journal of Drug Targeting, vol. 16, no. 4, pp. 282-293. https://doi.org/10.1080/10611860801900462

PLGA microspheres for the delivery of a novel subunit TB vaccine. / Kirby, Daniel J.; Rosenkrands, Ida; Agger, Else M.; Andersen, Peter; Coombes, Allan G.A.; Perrie, Yvonne.

In: Journal of Drug Targeting, Vol. 16, No. 4, 05.2008, p. 282-293.

Research output: Contribution to journalArticle

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