Polarization-correlation optical microscopy of anisotropic biological layers

A. G. Ushenko, A. V. Dubolazov, V. A. Ushenko, Yu A. Ushenko, M. Yu Sakhnovskiy, V. N. Balazyuk, O. Khukhlina, K. Viligorska, A. Bykov, A. Doronin, I. Meglinski

Research output: Contribution to journalConference article

Abstract

The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter - complex degree of mutual anisotropy - is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states - dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st - 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.

Original languageEnglish
Article number99712C
JournalProceedings of SPIE - International Society for Optical Engineering
Volume9971
DOIs
Publication statusPublished - 28 Sep 2016
EventApplications of Digital Image Processing XXXIX - San Diego, United States
Duration: 29 Aug 20161 Sep 2016

Fingerprint

myocardium
Microscopy
Optical microscopy
Myocardium
Anisotropy
Polarization
microscopy
anisotropy
Birefringence
polarization
Tissue
birefringence
heart diseases
myosins
Coronary Heart Disease
distribution moments
Myosin
Biopsy
Myosins
Interconnection

Bibliographical note

Copyright 2016 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

  • Fourier optics and signal processing
  • Imaging systems
  • Medical and biological imaging
  • Polarization

Cite this

Ushenko, A. G. ; Dubolazov, A. V. ; Ushenko, V. A. ; Ushenko, Yu A. ; Sakhnovskiy, M. Yu ; Balazyuk, V. N. ; Khukhlina, O. ; Viligorska, K. ; Bykov, A. ; Doronin, A. ; Meglinski, I. / Polarization-correlation optical microscopy of anisotropic biological layers. In: Proceedings of SPIE - International Society for Optical Engineering. 2016 ; Vol. 9971.
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title = "Polarization-correlation optical microscopy of anisotropic biological layers",
abstract = "The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter - complex degree of mutual anisotropy - is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states - dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st - 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.",
keywords = "Fourier optics and signal processing, Imaging systems, Medical and biological imaging, Polarization",
author = "Ushenko, {A. G.} and Dubolazov, {A. V.} and Ushenko, {V. A.} and Ushenko, {Yu A.} and Sakhnovskiy, {M. Yu} and Balazyuk, {V. N.} and O. Khukhlina and K. Viligorska and A. Bykov and A. Doronin and I. Meglinski",
note = "Copyright 2016 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.",
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Ushenko, AG, Dubolazov, AV, Ushenko, VA, Ushenko, YA, Sakhnovskiy, MY, Balazyuk, VN, Khukhlina, O, Viligorska, K, Bykov, A, Doronin, A & Meglinski, I 2016, 'Polarization-correlation optical microscopy of anisotropic biological layers', Proceedings of SPIE - International Society for Optical Engineering, vol. 9971, 99712C. https://doi.org/10.1117/12.2237926

Polarization-correlation optical microscopy of anisotropic biological layers. / Ushenko, A. G.; Dubolazov, A. V.; Ushenko, V. A.; Ushenko, Yu A.; Sakhnovskiy, M. Yu; Balazyuk, V. N.; Khukhlina, O.; Viligorska, K.; Bykov, A.; Doronin, A.; Meglinski, I.

In: Proceedings of SPIE - International Society for Optical Engineering, Vol. 9971, 99712C, 28.09.2016.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Polarization-correlation optical microscopy of anisotropic biological layers

AU - Ushenko, A. G.

AU - Dubolazov, A. V.

AU - Ushenko, V. A.

AU - Ushenko, Yu A.

AU - Sakhnovskiy, M. Yu

AU - Balazyuk, V. N.

AU - Khukhlina, O.

AU - Viligorska, K.

AU - Bykov, A.

AU - Doronin, A.

AU - Meglinski, I.

N1 - Copyright 2016 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2016/9/28

Y1 - 2016/9/28

N2 - The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter - complex degree of mutual anisotropy - is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states - dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st - 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.

AB - The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter - complex degree of mutual anisotropy - is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states - dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st - 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.

KW - Fourier optics and signal processing

KW - Imaging systems

KW - Medical and biological imaging

KW - Polarization

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U2 - 10.1117/12.2237926

DO - 10.1117/12.2237926

M3 - Conference article

AN - SCOPUS:85006922953

VL - 9971

JO - Proceedings of SPIE - International Society for Optical Engineering

JF - Proceedings of SPIE - International Society for Optical Engineering

SN - 0277-786X

M1 - 99712C

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