Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

Ernesto Tinajero-Díaz; Nicola Judge; Bo Li; Thomas Leigh; Robert D. Murphy; Paul D. Topham; Matthew J. Derry; Andreas Heise

doi:10.1021/acsmacrolett.4c00400

Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

Ernesto Tinajero-Díaz, Nicola Judge, Bo Li, Thomas Leigh, Robert D. Murphy, Paul D. Topham, Matthew J. Derry, Andreas Heise

Department of Chemistry, RCSI University of Medicine and Health Sciences, 123 St. Stephen’s Green, D02 YN77 Dublin, Ireland
AMBER, The SFI Advanced Materials and Bioengineering Research Centre, D02 YN77 Dublin, Ireland

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

3 Downloads (Pure)

Abstract

Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.

Original language	English
Pages (from-to)	1031-1036
Number of pages	6
Journal	ACS Macro Letters
Volume	13
Issue number	8
Early online date	29 Jul 2024
DOIs	https://doi.org/10.1021/acsmacrolett.4c00400
Publication status	Published - 20 Aug 2024

Bibliographical note

Data Access Statement

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsmacrolett.4c00400.

Funding

E.T.-D. acknowledges funding through the postdoctoral grant Margarita Salas awarded in 2021 by Spanish Ministry of Universities. This project has received funding from the European Union\u2019s Horizon 2020 research and innovation program under the Marie Sk\u0142odowska-Curie Grant Agreements 814236 (N.J.), 101103989 (T.L.), and 842599 (R.D.M.). This work was carried out with the support of Diamond Light Source, Instrument I22 (Proposal sm33098), and we acknowledge Dr Andy Smith and all I22 beamline staff for the support we received during the experiment.

Funders	Funder number
Spanish Ministry of Universities
Margarita Salas
Horizon 2020 Framework Programme	814236, 101103989, 842599

Access to Document

10.1021/acsmacrolett.4c00400Licence: CC BY 4.0

tinajero-díaz-et-al-2024-poly(l-proline)-stabilized-polypeptide-nanostructures-via-ring-opening-polymerization-induced
Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/).
Final published version, 4.06 MBLicence: CC BY 4.0

Cite this

@article{f21af056f19d49b1bd717da513f90082,

title = "Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)",

abstract = "Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.",

author = "Ernesto Tinajero-D{\'i}az and Nicola Judge and Bo Li and Thomas Leigh and Murphy, {Robert D.} and Topham, {Paul D.} and Derry, {Matthew J.} and Andreas Heise",

note = "Copyright {\textcopyright} 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/). ",

year = "2024",

month = aug,

day = "20",

doi = "10.1021/acsmacrolett.4c00400",

language = "English",

volume = "13",

pages = "1031--1036",

journal = "ACS Macro Letters",

issn = "2161-1653",

number = "8",

}

TY - JOUR

T1 - Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

AU - Tinajero-Díaz, Ernesto

AU - Judge, Nicola

AU - Li, Bo

AU - Leigh, Thomas

AU - Murphy, Robert D.

AU - Topham, Paul D.

AU - Derry, Matthew J.

AU - Heise, Andreas

PY - 2024/8/20

Y1 - 2024/8/20

N2 - Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.

AB - Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.

UR - https://pubs.acs.org/doi/10.1021/acsmacrolett.4c00400

UR - http://www.scopus.com/inward/record.url?scp=85199902108&partnerID=8YFLogxK

U2 - 10.1021/acsmacrolett.4c00400

DO - 10.1021/acsmacrolett.4c00400

M3 - Article

C2 - 39074359

SN - 2161-1653

VL - 13

SP - 1031

EP - 1036

JO - ACS Macro Letters

JF - ACS Macro Letters

IS - 8

ER -

Poly(l-proline)-Stabilized Polypeptide Nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

Abstract

Bibliographical note

Data Access Statement

Funding

Access to Document

Other files and links

Fingerprint

Cite this