PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Anupama Chembath; Mohammed Ashraf; Ben Wagstaffe; Yvonne  Stark; Erich Michel; Alina Rohrbach; Josef Kynast; Florian  Gisdon; Merve  Ayyildiz; Birte  Höcker; Andreas Plückthun; Anna V. Hine

PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Anupama Chembath
, Mohammed Ashraf
, Ben Wagstaffe
, Yvonne Stark
, Erich Michel
, Alina Rohrbach
, Josef Kynast
, Florian Gisdon
, Merve Ayyildiz
, Birte Höcker
, Andreas Plückthun
, Anna V. Hine^*

^*Corresponding author for this work

Aston University

Department of Biochemistry , University of Zurich , CH-8057 Zürich , Switzerland
Department of Biochemistry , University of Bayreuth , D-95447 Bayreuth , Germany

Research output: Unpublished contribution to conference › Poster › peer-review

Abstract

PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat
proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

Original language	English
Publication status	Published - 4 May 2022
Event	PEGS Boston - The essential protein engineering and cell therapy summit - Boston, Boston, United States Duration: 2 May 2022 → 6 May 2022 https://www.pegsummit.com/

Conference

Conference	PEGS Boston - The essential protein engineering and cell therapy summit
Abbreviated title	PEGS Boston 2022
Country/Territory	United States
City	Boston
Period	2/05/22 → 6/05/22
Internet address	https://www.pegsummit.com/

Access to Document

https://www.pegsummit.com/poster-titles

1 Article

Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules
Stark, Y., Menard, F., Jeliazkov, J., Ernst, P., Chembath, A., Ashraf, M., Hine, A. V. & Plueckthun, A., 2 Jul 2024, In: Proceedings of the National Academy of Sciences of the United States of America. 121, 27, 11 p., e2318198121.
Research output: Contribution to journal › Article › peer-review

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Chembath, A., Ashraf, M., Wagstaffe, B., Stark, Y., Michel, E., Rohrbach, A., Kynast, J., Gisdon, F., Ayyildiz, M., Höcker, B., Plückthun, A., & Hine, A. V. (2022). PRe-ART - Creating Synthetic Replacements for Reagent Antibodies. Poster session presented at PEGS Boston - The essential protein engineering and cell therapy summit, Boston, Massachusetts, United States. https://www.pegsummit.com/poster-titles

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title = "PRe-ART - Creating Synthetic Replacements for Reagent Antibodies",

abstract = "PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.",

author = "Anupama Chembath and Mohammed Ashraf and Ben Wagstaffe and Yvonne Stark and Erich Michel and Alina Rohrbach and Josef Kynast and Florian Gisdon and Merve Ayyildiz and Birte H{\"o}cker and Andreas Pl{\"u}ckthun and Hine, \{Anna V.\}",

year = "2022",

month = may,

day = "4",

language = "English",

note = " PEGS Boston - The essential protein engineering and cell therapy summit, PEGS Boston 2022 ; Conference date: 02-05-2022 Through 06-05-2022",

url = "https://www.pegsummit.com/",

}

Chembath, A , Ashraf, M, Wagstaffe, B, Stark, Y, Michel, E, Rohrbach, A, Kynast, J, Gisdon, F, Ayyildiz, M, Höcker, B, Plückthun, A & Hine, AV 2022, 'PRe-ART - Creating Synthetic Replacements for Reagent Antibodies', PEGS Boston - The essential protein engineering and cell therapy summit, Boston, United States, 2/05/22 - 6/05/22. <https://www.pegsummit.com/poster-titles>

TY - CONF

T1 - PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

AU - Chembath, Anupama

AU - Ashraf, Mohammed

AU - Wagstaffe, Ben

AU - Stark, Yvonne

AU - Michel, Erich

AU - Rohrbach, Alina

AU - Kynast, Josef

AU - Gisdon, Florian

AU - Ayyildiz, Merve

AU - Höcker, Birte

AU - Plückthun, Andreas

AU - Hine, Anna V.

PY - 2022/5/4

Y1 - 2022/5/4

N2 - PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

AB - PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

M3 - Poster

T2 - PEGS Boston - The essential protein engineering and cell therapy summit

Y2 - 2 May 2022 through 6 May 2022

ER -

PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Abstract

Conference

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Research output

Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules

Cite this