PRe-ART: Predictive Reagent Antibody Replacement Technology

Anupama Chembath, Ben Wagstaffe, Mohammed Ashraf, Erich Michel, Yvonne Stark, Noelia Ferruz-Capapey, Josef Kynast, Birte Höcker, Anna V. Hine*, Andreas Plückthun

*Corresponding author for this work

Research output: Contribution to conferenceAbstract

Abstract

Reagent antibodies are used extensively in research, typically to bind or capture protein targets. Unlike well-characterised therapeutic antibodies, about half of the commercially available reagent antibodies have previously been shown to not function correctly either in terms of their specificity or else in recognising their target at all. PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace conventional reagent antibodies with sequence-defined, designed armadillo repeat proteins (dArmRPs), capable of conserved and specific binding of extended peptides. PRe-ART uses a feedback loop of experimental synthesis and evaluation with computational modelling to iteratively develop the properties of novel dArmRPs. Ultimately, PRe-ART aims to create an encyclopaedia of pre-designed and experimentally pre-selected modules that may be combined to generate unique binding molecules that function as antibody replacements. Progress to date in terms of library synthesis, screening and modelling will be presented.
Original languageEnglish
Publication statusPublished - 18 Nov 2019
EventPEGS Europe 2019: 11th Annual Protein and Antibody Engineering Summit - Lisbon Congress Centre, Lisbon, Portugal
Duration: 18 Nov 201922 Nov 2019
https://www.pegsummiteurope.com/

Conference

ConferencePEGS Europe 2019
CountryPortugal
CityLisbon
Period18/11/1922/11/19
Internet address

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