Prevalence and Virulence of Commensal Pseudomonas Aeruginosa Isolates from Healthy Individuals in Southern Vietnam (2018-2020)

Nguyen Bao Vy Tran, Quang Minh Truong, Lam Que Anh Nguyen, Ngoc My Huong Nguyen, Quang Hung Tran, Thi Tuyet Phuong Dinh, Vinh Son Hua, Van Dung Nguyen, Peter A. Lambert, Thi Thu Hoai Nguyen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Understanding the colonization of Pseudomonas aeruginosa (P. aeruginosa) in healthy humans is useful for future prevention and treatment of P. aeruginosa infection. This study aimed to investigate the prevalence and risk factors of of P. aeruginosa colonization in healthy humans. At the same time, the virulence of the isolated P. aeruginosa was also studied. In the study, 609 Vietnamese volunteers (310 females and 299 males, age range of 2 to 73 years), who had no acute infection or disease symptoms participated at the time of sample collection. Samples were taken from the throat, nostrils, and outer ears. P. aeruginosa was found in 19 participants (3.12%, 95% CI: 0.017−0.045), mainly from the throat (11/19, 57.89%). Participants with a history of sinusitis were 11.57 times more likely to be colonized with P. aeruginosa than participants without a history of sinusitis (OR: 11.57, 95% CI: 4.08−32.76, p-value < 0.0001, Fisher’s Exact test). Age and sex were not significantly associated with P. aeruginosa colonization. Among 16 P. aeruginosa isolates used in virulence tests, 100% (16/16) were positive for the synthesis of biofilm, pyocyanin, and siderophores; 93.75% (15/16) isolates were positive for the synthesis of gelatinase and protease; and 50% (8/16) isolates were positive for lipase. There were no differences in the pattern and range of virulence factors of P. aeruginosa isolates taken from participants with and without sinusitis history. P. aeruginosa colonized 3.12% of participants, and its presence was associated with sinusitis history.

Original languageEnglish
Article number54
Number of pages11
Issue number1
Early online date26 Dec 2022
Publication statusPublished - Jan 2023

Bibliographical note

Funding Information:
This research is funded by Vietnam National University of Ho Chi Minh City under grant number C2020-28-03.

Publisher Copyright:
Copyright © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (


  • colonization risk factors
  • commensal Pseudomonas aeruginosa
  • sinusitis
  • virulence


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