Probing molecular changes induced in DNA by reactive oxygen species with monoclonal antibodies

Susan Blount, Helen R. Griffiths, N.A. Staines, Joseph Lunec

Research output: Contribution to journalArticle

Abstract

Antibodies reactive with native double stranded DNA are characteristic of the chronic inflammatory disease systemic lupus erythematosus. Native DNA is however, a poor immunogen and the mechanism of anti-DNA antibody production is incompletely understood. Modification of DNA can increase its immunogenicity and in inflammatory disease states reactive oxygen species produced from phagocytic cells have been shown to thus modify DNA. In this study, monoclonal antibodies produced spontaneously by two mice strains with lupus-like disease were used in a competition ELISA to monitor changes to DNA induced by reactive oxygen species. Different procedures for reactive oxygen species generation were found to cause distinct and characteristic changes to DNA involving modifications of base residues, the sugar-phosphate backbone and the gross conformational structure of double-stranded DNA. In view of this, it may be possible to use these antibodies further to probe DNA and infer the source and nature of the reactive oxygen species it has been exposed to, particularly in vivo.
Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalImmunology Letters
Volume34
Issue number2
DOIs
Publication statusPublished - Oct 1992

Keywords

  • monoclonal antibody
  • DNA
  • reactive oxygen species

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