Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Carmen Hierro-Iglesias; Cornelius O. Fatokun; Annie Chimphango; Richard Bayitse; Paula Helena Blanco Sanchez; Patricia Thornley; Alfred Fernandez-Castane

doi:10.1016/j.jece.2023.111815

Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Carmen Hierro-Iglesias
, Cornelius O. Fatokun
, Annie Chimphango
, Richard Bayitse
, Paula Helena Blanco Sanchez
, Patricia Thornley
, Alfred Fernandez-Castane^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

22 Downloads (Pure)

Abstract

Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

Original language	English
Article number	111815
Number of pages	13
Journal	Journal of Environmental Chemical engineering
Volume	12
Issue number	1
Early online date	25 Dec 2023
DOIs	https://doi.org/10.1016/j.jece.2023.111815
Publication status	Published - Feb 2024

Bibliographical note

This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Acid hydrolysis
Cassava
Cupriavidus necator
Polyhydroxyalkanoates
Waste valorization

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10.1016/j.jece.2023.111815Licence: CC BY 4.0

1-s2.0-S221334372302554X-main
This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Cite this

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title = "Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates",

abstract = "Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97\% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31\% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.",

keywords = "Acid hydrolysis, Cassava, Cupriavidus necator, Polyhydroxyalkanoates, Waste valorization",

author = "Carmen Hierro-Iglesias and Fatokun, \{Cornelius O.\} and Annie Chimphango and Richard Bayitse and \{Blanco Sanchez\}, \{Paula Helena\} and Patricia Thornley and Alfred Fernandez-Castane",

note = "This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",

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T1 - Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

AU - Hierro-Iglesias, Carmen

AU - Fatokun, Cornelius O.

AU - Chimphango, Annie

AU - Bayitse, Richard

AU - Blanco Sanchez, Paula Helena

AU - Thornley, Patricia

AU - Fernandez-Castane, Alfred

N1 - This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2024/2

Y1 - 2024/2

N2 - Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

AB - Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

KW - Acid hydrolysis

KW - Cassava

KW - Cupriavidus necator

KW - Polyhydroxyalkanoates

KW - Waste valorization

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JF - Journal of Environmental Chemical engineering

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Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Abstract

Bibliographical note

UN SDGs

Keywords

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