Production of recombinant G protein-coupled receptor in yeast for structural and functional analysis

Richard A.J. Darby; Mohammed Jamshad; Ljuban Grgic; William J. Holmes; Roslyn M. Bill

doi:10.1002/9780470749210.ch5

Production of recombinant G protein-coupled receptor in yeast for structural and functional analysis

Richard A.J. Darby^*, Mohammed Jamshad, Ljuban Grgic, William J. Holmes, Roslyn M. Bill

^*Corresponding author for this work

Research output: Chapter in Book/Published conference output › Chapter

Original language	English
Title of host publication	G protein-coupled receptors
Subtitle of host publication	essential methods
Editors	David R. Poyner, Mark Wheatley
Publisher	Wiley-Blackwell
Pages	85-110
Number of pages	26
ISBN (Electronic)	978-0-470-74921-0
ISBN (Print)	978-0-470-74914-2
DOIs	https://doi.org/10.1002/9780470749210.ch5
Publication status	Published - 2010

Keywords

analytical ultracentrifugation (AUC), technique characterizing solution state of macromolecules
DNA sequence encoding GPCR of interest - amplified via polymerase chain reaction (PCR)
G protein-coupled receptors (GPCRs) - naturally present in native membranes
PCR amplification and cloning
preparation of expression plasmids
protocol 5.2 yeast transformations
protocol 5.3 screening for GPCR production in P. pastoris clones
pProtocol 5.5 GPCR solubilization and purification
recombinant G protein-coupled receptor production in yeast - structural and functional analysis
solubilization and purification

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10.1002/9780470749210.ch5

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title = "Production of recombinant G protein-coupled receptor in yeast for structural and functional analysis",

keywords = "analytical ultracentrifugation (AUC), technique characterizing solution state of macromolecules, DNA sequence encoding GPCR of interest - amplified via polymerase chain reaction (PCR), G protein-coupled receptors (GPCRs) - naturally present in native membranes, PCR amplification and cloning, preparation of expression plasmids, protocol 5.2 yeast transformations, protocol 5.3 screening for GPCR production in P. pastoris clones, pProtocol 5.5 GPCR solubilization and purification, recombinant G protein-coupled receptor production in yeast - structural and functional analysis, solubilization and purification",

author = "Darby, {Richard A.J.} and Mohammed Jamshad and Ljuban Grgic and Holmes, {William J.} and Bill, {Roslyn M.}",

year = "2010",

doi = "10.1002/9780470749210.ch5",

language = "English",

isbn = "978-0-470-74914-2",

pages = "85--110",

editor = "Poyner, {David R.} and Mark Wheatley",

booktitle = "G protein-coupled receptors",

publisher = "Wiley-Blackwell",

address = "United States",

}

TY - CHAP

T1 - Production of recombinant G protein-coupled receptor in yeast for structural and functional analysis

AU - Darby, Richard A.J.

AU - Jamshad, Mohammed

AU - Grgic, Ljuban

AU - Holmes, William J.

AU - Bill, Roslyn M.

PY - 2010

Y1 - 2010

KW - analytical ultracentrifugation (AUC), technique characterizing solution state of macromolecules

KW - DNA sequence encoding GPCR of interest - amplified via polymerase chain reaction (PCR)

KW - G protein-coupled receptors (GPCRs) - naturally present in native membranes

KW - PCR amplification and cloning

KW - preparation of expression plasmids

KW - protocol 5.2 yeast transformations

KW - protocol 5.3 screening for GPCR production in P. pastoris clones

KW - pProtocol 5.5 GPCR solubilization and purification

KW - recombinant G protein-coupled receptor production in yeast - structural and functional analysis

KW - solubilization and purification

UR - http://www.scopus.com/inward/record.url?scp=84886322718&partnerID=8YFLogxK

UR - http://onlinelibrary.wiley.com/doi/10.1002/9780470749210.ch5/summary

U2 - 10.1002/9780470749210.ch5

DO - 10.1002/9780470749210.ch5

M3 - Chapter

AN - SCOPUS:84886322718

SN - 978-0-470-74914-2

SP - 85

EP - 110

BT - G protein-coupled receptors

A2 - Poyner, David R.

A2 - Wheatley, Mark

PB - Wiley-Blackwell

ER -

Production of recombinant G protein-coupled receptor in yeast for structural and functional analysis

Keywords

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G protein-coupled receptors: essential methods

Preface

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