| Original language | English |
|---|---|
| Title of host publication | G protein-coupled receptors |
| Subtitle of host publication | essential methods |
| Editors | David R. Poyner, Mark Wheatley |
| Publisher | Wiley-Blackwell |
| Pages | 85-110 |
| Number of pages | 26 |
| ISBN (Electronic) | 978-0-470-74921-0 |
| ISBN (Print) | 978-0-470-74914-2 |
| DOIs | |
| Publication status | Published - 2010 |
Keywords
- analytical ultracentrifugation (AUC), technique characterizing solution state of macromolecules
- DNA sequence encoding GPCR of interest - amplified via polymerase chain reaction (PCR)
- G protein-coupled receptors (GPCRs) - naturally present in native membranes
- PCR amplification and cloning
- preparation of expression plasmids
- protocol 5.2 yeast transformations
- protocol 5.3 screening for GPCR production in P. pastoris clones
- pProtocol 5.5 GPCR solubilization and purification
- recombinant G protein-coupled receptor production in yeast - structural and functional analysis
- solubilization and purification
-
G protein-coupled receptors: essential methods
Poyner, D. R. (Editor) & Wheatley, M. (Editor), 25 Jan 2010, Wiley-Blackwell.Research output: Book/Report › Book
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Preface
Poyner, D. R. & Wheatley, M., 2010, G protein-coupled receptors: essential methods. Poyner, D. R. & Wheatley, M. (eds.). Wiley-BlackwellResearch output: Chapter in Book/Published conference output › Foreword/postscript
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