Proteomic analysis of a noninvasive human model of acute inflammation and its resolution

the twenty-one day gingivitis model

Melissa M. Grant, Andrew J. Creese, Gordon Barr, Martin R. Ling, Ann E. Scott, John B. Matthews, Helen R Griffiths, Helen J. Cooper, Iain L.C. Chapple

Research output: Contribution to journalArticle

Abstract

The 21-day experimental gingivitis model, an established noninvasive model of inflammation in response to increasing bacterial accumulation in humans, is designed to enable the study of both the induction and resolution of inflammation. Here, we have analyzed gingival crevicular fluid, an oral fluid comprising a serum transudate and tissue exudates, by LC-MS/MS using Fourier transform ion cyclotron resonance mass spectrometry and iTRAQ isobaric mass tags, to establish meta-proteomic profiles of inflammation-induced changes in proteins in healthy young volunteers. Across the course of experimentally induced gingivitis, we identified 16 bacterial and 186 human proteins. Although abundances of the bacterial proteins identified did not vary temporally, Fusobacterium outer membrane proteins were detected. Fusobacterium species have previously been associated with periodontal health or disease. The human proteins identified spanned a wide range of compartments (both extracellular and intracellular) and functions, including serum proteins, proteins displaying antibacterial properties, and proteins with functions associated with cellular transcription, DNA binding, the cytoskeleton, cell adhesion, and cilia. PolySNAP3 clustering software was used in a multilayered analytical approach. Clusters of proteins that associated with changes to the clinical parameters included neuronal and synapse associated proteins.
Original languageEnglish
Pages (from-to)4732-4744
Number of pages13
JournalJournal of Proteome Research
Volume9
Issue number9
DOIs
Publication statusPublished - 27 Jul 2010

Fingerprint

Gingivitis
Proteomics
Inflammation
Fusobacterium
Proteins
Gingival Crevicular Fluid
Cyclotrons
Cyclotron resonance
Bacterial Proteins
Fluids
Cilia
Cell adhesion
Exudates and Transudates
Fourier Analysis
Transcription
Cytoskeleton
Cell Adhesion
Synapses
Mass spectrometry
Cluster Analysis

Bibliographical note

All articles with an ACS AuthorChoice logo published prior to April 1, 2013, are subject to the ACS AuthorChoice/ACS Editors’ Choice Terms and Conditions. © 2010 American Chemical Society

Keywords

  • acute disease
  • adult
  • liquid chromatography
  • cluster analysis
  • female
  • fusobacterium
  • gingival crevicular fluid
  • gingivitis
  • humans
  • inflammation
  • isotope labeling
  • male
  • metagenome
  • biological models
  • proteome
  • proteomics
  • Fourier transform infrared spectroscopy
  • nonparametric statistics
  • tandem mass spectrometry

Cite this

Grant, M. M., Creese, A. J., Barr, G., Ling, M. R., Scott, A. E., Matthews, J. B., ... Chapple, I. L. C. (2010). Proteomic analysis of a noninvasive human model of acute inflammation and its resolution: the twenty-one day gingivitis model. Journal of Proteome Research, 9(9), 4732-4744. https://doi.org/10.1021/pr100446f
Grant, Melissa M. ; Creese, Andrew J. ; Barr, Gordon ; Ling, Martin R. ; Scott, Ann E. ; Matthews, John B. ; Griffiths, Helen R ; Cooper, Helen J. ; Chapple, Iain L.C. / Proteomic analysis of a noninvasive human model of acute inflammation and its resolution : the twenty-one day gingivitis model. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 9. pp. 4732-4744.
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Grant, MM, Creese, AJ, Barr, G, Ling, MR, Scott, AE, Matthews, JB, Griffiths, HR, Cooper, HJ & Chapple, ILC 2010, 'Proteomic analysis of a noninvasive human model of acute inflammation and its resolution: the twenty-one day gingivitis model', Journal of Proteome Research, vol. 9, no. 9, pp. 4732-4744. https://doi.org/10.1021/pr100446f

Proteomic analysis of a noninvasive human model of acute inflammation and its resolution : the twenty-one day gingivitis model. / Grant, Melissa M.; Creese, Andrew J.; Barr, Gordon; Ling, Martin R.; Scott, Ann E.; Matthews, John B.; Griffiths, Helen R; Cooper, Helen J.; Chapple, Iain L.C.

In: Journal of Proteome Research, Vol. 9, No. 9, 27.07.2010, p. 4732-4744.

Research output: Contribution to journalArticle

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