The G-protein coupled receptor (GPCR) superfamily fulfils various metabolic functions and interacts with a diverse range of ligands. There is a lack of sequence similarity between the six classes that comprise the GPCR superfamily. Moreover, most novel GPCRs found have low sequence similarity to other family members which makes it difficult to infer properties from related receptors. Many different approaches have been taken towards developing efficient and accurate methods for GPCR classification, ranging from motif-based systems to machine learning as well as a variety of alignment-free techniques based on the physiochemical properties of their amino acid sequences. This review describes the inherent difficulties in developing a GPCR classification algorithm and includes techniques previously employed in this area.
Davies, M. N., Gloriam, D. E., Secker, A., Freitas, A. A., Mendao, M., Timmis, J., & Flower, D. R. (2007). Proteomic applications of automated GPCR classification. Proteomics, 7(16), 2800-2814. https://doi.org/10.1002/pmic.200700093