Providing osteogenesis conditions to mesenchymal stem cells using bioactive nanocomposite bone scaffolds

Jung Ju Kim, Guang Zhen Jin, Hye Sun Yu, Seong Jun Choi, Hae Won Kim*, Ivan B. Wall

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Development of bone scaffolds with excellent osteogenic potential is highly important for stem cell-based bone engineering. Here we developed novel scaffolds made of poly(lactic acid) (PLA) biopolymer with bioactive glass nanocomponent. In vitro bone bioactivity and osteogenic potential of the nanocomposite scaffolds were determined using bone marrow mesenchymal stem cells. Glass nanocomponent was evenly embedded within the PLA matrix while preserving the scaffold pore structure. Simulated body fluid (SBF) test revealed rapid induction of bone mineral-like apatite over the surface of the nanocomposite scaffold, which was not readily observed in the PLA. Cells adhered well onto the nanocomposite scaffold and multiplied during culture period. Nanocomposite scaffold significantly stimulated alkaline phosphatase (ALP) activity and the expression of bone-associated genes (collagen I, ALP, osteopontin and osteocalcin) with respect to PLA. Western blot analysis confirmed the osteogenic protein level was also higher on the nanocomposite scaffold. Results suggest that the nanocomposite scaffolds provide favorable conditions for osteogenesis of MSCs and thus find potential uses in bone tissue engineering.

Original languageEnglish
Pages (from-to)2545-2551
Number of pages7
JournalMaterials Science and Engineering C
Volume32
Issue number8
DOIs
Publication statusPublished - 1 Dec 2012

Keywords

  • Bioactive glass
  • Bone tissue engineering
  • Mesenchymal stem cells
  • Nanocomposite scaffolds
  • Osteogenic differentiation

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