ProxiMAX and MAX randomization: Precision protein engineering

Anna V. Hine, Marta Ferreira Amaral, Anupama Chembath, Ben Wagstaffe

Research output: Contribution to conferencePoster

Abstract

‘ProxiMAX’ and ‘MAX’ randomization technologies are defined saturation mutagenesis processes that deliver precision control of both identity and relative ratio of amino acids at specified locations within a protein library. Both processes are non-degenerate, meaning that encoding DNA libraries are as small as is physically possible. ProxiMAX is the technology that lies behind Isogenica’s Colibra™ offering and is ideal for saturating contiguous codons, as required in antibody libraries. In contrast, ‘MAX’ randomization targets codons at disparate locations within a gene and is therefore more applicable to other scaffolds or proteins. Since no constraints are imposed by the genetic code, both technologies can eliminate unwanted amino acids such as cysteine and methionine from libraries or encode desired subsets of amino acids with ease. Yet their underlying processes are quite different. This presentation will examine the development of both ProxiMAX and MAX randomization process and give examples of the high quality libraries created to date using both techniques.
Original languageEnglish
Publication statusPublished - 2018
EventSynthetic Biology UK 2018 - We the Curious, Bristol, Bristol, United Kingdom
Duration: 19 Nov 201820 Nov 2018
https://www.eventsforce.net/biochemsoc/frontend/reg/thome.csp?pageID=18045&ef_sel_menu=224&eventID=43

Conference

ConferenceSynthetic Biology UK 2018
Abbreviated titleSBUK
CountryUnited Kingdom
CityBristol
Period19/11/1820/11/18
Internet address

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engineering

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Hine, A. V., Ferreira Amaral, M., Chembath, A., & Wagstaffe, B. (2018). ProxiMAX and MAX randomization: Precision protein engineering. Poster session presented at Synthetic Biology UK 2018, Bristol, United Kingdom.
Hine, Anna V. ; Ferreira Amaral, Marta ; Chembath, Anupama ; Wagstaffe, Ben. / ProxiMAX and MAX randomization: Precision protein engineering. Poster session presented at Synthetic Biology UK 2018, Bristol, United Kingdom.
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Hine, AV, Ferreira Amaral, M, Chembath, A & Wagstaffe, B 2018, 'ProxiMAX and MAX randomization: Precision protein engineering' Synthetic Biology UK 2018, Bristol, United Kingdom, 19/11/18 - 20/11/18, .

ProxiMAX and MAX randomization: Precision protein engineering. / Hine, Anna V.; Ferreira Amaral, Marta; Chembath, Anupama; Wagstaffe, Ben.

2018. Poster session presented at Synthetic Biology UK 2018, Bristol, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - ProxiMAX and MAX randomization: Precision protein engineering

AU - Hine, Anna V.

AU - Ferreira Amaral, Marta

AU - Chembath, Anupama

AU - Wagstaffe, Ben

PY - 2018

Y1 - 2018

N2 - ‘ProxiMAX’ and ‘MAX’ randomization technologies are defined saturation mutagenesis processes that deliver precision control of both identity and relative ratio of amino acids at specified locations within a protein library. Both processes are non-degenerate, meaning that encoding DNA libraries are as small as is physically possible. ProxiMAX is the technology that lies behind Isogenica’s Colibra™ offering and is ideal for saturating contiguous codons, as required in antibody libraries. In contrast, ‘MAX’ randomization targets codons at disparate locations within a gene and is therefore more applicable to other scaffolds or proteins. Since no constraints are imposed by the genetic code, both technologies can eliminate unwanted amino acids such as cysteine and methionine from libraries or encode desired subsets of amino acids with ease. Yet their underlying processes are quite different. This presentation will examine the development of both ProxiMAX and MAX randomization process and give examples of the high quality libraries created to date using both techniques.

AB - ‘ProxiMAX’ and ‘MAX’ randomization technologies are defined saturation mutagenesis processes that deliver precision control of both identity and relative ratio of amino acids at specified locations within a protein library. Both processes are non-degenerate, meaning that encoding DNA libraries are as small as is physically possible. ProxiMAX is the technology that lies behind Isogenica’s Colibra™ offering and is ideal for saturating contiguous codons, as required in antibody libraries. In contrast, ‘MAX’ randomization targets codons at disparate locations within a gene and is therefore more applicable to other scaffolds or proteins. Since no constraints are imposed by the genetic code, both technologies can eliminate unwanted amino acids such as cysteine and methionine from libraries or encode desired subsets of amino acids with ease. Yet their underlying processes are quite different. This presentation will examine the development of both ProxiMAX and MAX randomization process and give examples of the high quality libraries created to date using both techniques.

M3 - Poster

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Hine AV, Ferreira Amaral M, Chembath A, Wagstaffe B. ProxiMAX and MAX randomization: Precision protein engineering. 2018. Poster session presented at Synthetic Biology UK 2018, Bristol, United Kingdom.