TY - JOUR
T1 - Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli
AU - Calleja, Daniel
AU - Fernández-castañé, Alfred
AU - Pasini, Martina
AU - De Mas, Carles
AU - López-santín, Josep
PY - 2014/10/1
Y1 - 2014/10/1
N2 - An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 ΔglyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC–MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
AB - An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 ΔglyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC–MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
UR - https://www.sciencedirect.com/science/article/pii/S1369703X14002344
U2 - 10.1016/j.bej.2014.08.017
DO - 10.1016/j.bej.2014.08.017
M3 - Article
SN - 1369-703X
VL - 91
SP - 210
EP - 219
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
ER -