Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries

Alex Cheong; Samuel J. Fountain; David J. Beech

doi:10.1007/978-1-59745-526-8_2

Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries

Alex Cheong^*
, Samuel J. Fountain
, David J. Beech

^*Corresponding author for this work

University of Leeds

Research output: Chapter in Book/Published conference output › Chapter

1 Citation (Scopus)

Abstract

Voltage-gated K+ channels (K_V channels) are encoded by the KCNx gene family and have such a wide range of properties that it is necessary to identify the precise expression profile that is instrumental in governing the electrical phenotype of a cell and its response to extrinsic factors. Real-time quantitative RT-PCR methodology has been developed and validated for specific RNA species in vascular smooth muscle cells. We have shown that most of the KCNA gene family, encoding the major K_Vα1 subunits, was markedly up-regulated in the resistance artery compared to the thoracic aorta, in line with reported patch-clamp recordings. Thus quantitative real-time RT-PCR data can be translated into physiological response.

Original language	English
Title of host publication	Potassium Channels
Subtitle of host publication	Methods and Protocols
Editors	J.D Lippiat
Publisher	Humana Press
Pages	19-33
Number of pages	15
ISBN (Electronic)	978-1-59745-526-8
ISBN (Print)	9781934115657
DOIs	https://doi.org/10.1007/978-1-59745-526-8_2
Publication status	Published - 2008

Publication series

Name	Methods in Molecular Biology
Volume	491
ISSN (Print)	1064-3745

Keywords

Potassium channel
Real-time RT-PCR
Smooth muscle

Access to Document

10.1007/978-1-59745-526-8_2

Cite this

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title = "Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries",

abstract = "Voltage-gated K+ channels (KV channels) are encoded by the KCNx gene family and have such a wide range of properties that it is necessary to identify the precise expression profile that is instrumental in governing the electrical phenotype of a cell and its response to extrinsic factors. Real-time quantitative RT-PCR methodology has been developed and validated for specific RNA species in vascular smooth muscle cells. We have shown that most of the KCNA gene family, encoding the major KVα1 subunits, was markedly up-regulated in the resistance artery compared to the thoracic aorta, in line with reported patch-clamp recordings. Thus quantitative real-time RT-PCR data can be translated into physiological response.",

keywords = "Potassium channel, Real-time RT-PCR, Smooth muscle",

author = "Alex Cheong and Fountain, \{Samuel J.\} and Beech, \{David J.\}",

year = "2008",

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language = "English",

isbn = "9781934115657",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "19--33",

editor = "J.D Lippiat",

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address = "United States",

}

Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries. / Cheong, Alex; Fountain, Samuel J.; Beech, David J.
Potassium Channels: Methods and Protocols. ed. / J.D Lippiat. Humana Press, 2008. p. 19-33 (Methods in Molecular Biology; Vol. 491).

Research output: Chapter in Book/Published conference output › Chapter

TY - CHAP

T1 - Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries

AU - Cheong, Alex

AU - Fountain, Samuel J.

AU - Beech, David J.

PY - 2008

Y1 - 2008

N2 - Voltage-gated K+ channels (KV channels) are encoded by the KCNx gene family and have such a wide range of properties that it is necessary to identify the precise expression profile that is instrumental in governing the electrical phenotype of a cell and its response to extrinsic factors. Real-time quantitative RT-PCR methodology has been developed and validated for specific RNA species in vascular smooth muscle cells. We have shown that most of the KCNA gene family, encoding the major KVα1 subunits, was markedly up-regulated in the resistance artery compared to the thoracic aorta, in line with reported patch-clamp recordings. Thus quantitative real-time RT-PCR data can be translated into physiological response.

AB - Voltage-gated K+ channels (KV channels) are encoded by the KCNx gene family and have such a wide range of properties that it is necessary to identify the precise expression profile that is instrumental in governing the electrical phenotype of a cell and its response to extrinsic factors. Real-time quantitative RT-PCR methodology has been developed and validated for specific RNA species in vascular smooth muscle cells. We have shown that most of the KCNA gene family, encoding the major KVα1 subunits, was markedly up-regulated in the resistance artery compared to the thoracic aorta, in line with reported patch-clamp recordings. Thus quantitative real-time RT-PCR data can be translated into physiological response.

KW - Potassium channel

KW - Real-time RT-PCR

KW - Smooth muscle

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UR - https://link.springer.com/protocol/10.1007/978-1-59745-526-8_2

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AN - SCOPUS:60349103090

SN - 9781934115657

T3 - Methods in Molecular Biology

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BT - Potassium Channels

A2 - Lippiat, J.D

PB - Humana Press

ER -

Quantitative RT-PCR methods for investigation of low copy potassium channel gene expression in native murine arteries

Abstract

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Keywords

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