RAMPs and CGRP receptors

James Barwell, Denise Wootten, John Simms, Debbie L. Hay, David R. Poyner

Research output: Contribution to journalArticle

Abstract

Receptor activity modifying protein 1 (RAMP1) forms a complex with calcitonin receptor-like receptor (CLR) to produce the receptor for calcitonin gene-related peptide (CGRP). RAMP1 has two main roles. It facilitates the cell-surface expression of CLR. It is also essential for the binding of CGRP to the receptor. It seems likely that Y66, F93, H97 and F101, amongst other residues, form a binding site for CLR. These cluster together on the same face of the extracellular portion of RAMP1, probably close to where it enters the plasma membrane. Residues at the other end of RAMP1 are most likely to be involved in CGRP recognition, although it is currently unclear how they do this. Within this area, W74 is important for the binding of the nonpeptide antagonist, BIBN4096BS, although it does not seem to be involved in the binding of CGRP itself. It has been shown that there is an epitope within residues 23-60 of CLR that are essential for RAMP recognition. Under some circumstances, changes in the expression of RAMP1 can alter the sensitivity of cells to CGRP, demonstrating that regulation of its levels may be of physiological or pathophysiological importance.
LanguageEnglish
Pages13-24
Number of pages12
JournalAdvances in Experimental Medicine and Biology
Volume744
DOIs
Publication statusPublished - 2012

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Receptor Activity-Modifying Protein 1
Calcitonin Gene-Related Peptide Receptors
Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide
Cell membranes
Epitopes
Binding Sites
Cell Membrane

Cite this

Barwell, James ; Wootten, Denise ; Simms, John ; Hay, Debbie L. ; Poyner, David R. / RAMPs and CGRP receptors. In: Advances in Experimental Medicine and Biology. 2012 ; Vol. 744. pp. 13-24.
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RAMPs and CGRP receptors. / Barwell, James; Wootten, Denise; Simms, John; Hay, Debbie L.; Poyner, David R.

In: Advances in Experimental Medicine and Biology, Vol. 744, 2012, p. 13-24.

Research output: Contribution to journalArticle

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