RAPD for the typing of coagulase-negative staphylococci implicated in catheter-related bloodstream infection

A.L. Casey, T. Worthington, J.M. Caddick, A.C. Hilton, P.A. Lambert, T.S.J. Elliott

Research output: Contribution to journalArticle

Abstract

Objectives: A rapid random amplification of polymorphic DNA (RAPD) technique was developed to distinguish between strains of coagulase-negative staphylococci (CoNS) involved in central venous catheter (CVC)-related bloodstream infection. Its performance was compared with that of pulsed-field gel electrophoresis (PFGE). Methods: Patients at the University Hospital Birmingham NHS Foundation Trust, U.K. who underwent stem cell transplantation and were diagnosed with CVC-related bloodstream infection due to CoNS whilst on the bone marrow transplant unit were studied. Isolates of CoNS were genotyped by PFGE and RAPD, the latter employing a single primer and a simple DNA extraction method. Results: Both RAPD and PFGE were highly discriminatory (Simpson's diversity index, 0.96 and 0.99, respectively). Within the 49 isolates obtained from blood cultures of 33 patients, 20 distinct strains were identified by PFGE and 25 by RAPD. Of the 25 strains identified by RAPD, nine clusters of CoNS contained isolates from multiple patients, suggesting limited nosocomial spread. However, there was no significant association between time of inpatient stay and infection due to any particular strain. Conclusion: The RAPD technique presented allows CoNS strains to be genotyped with high discrimination within 4 h, facilitating real-time epidemiological investigations. In this study, no single strain of CoNS was associated with a significant number of CVC-related bloodstream infections. © 2005 Published by Elsevier Ltd on behalf of the British Infection Society.

Original languageEnglish
Pages (from-to)282-289
Number of pages8
JournalJournal of Infection
Volume52
Issue number4
DOIs
Publication statusPublished - Apr 2006

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Casey, A.L.; Worthington, Tony; Caddick, J.M.; Hilton, Anthony; Lambert, Peter A. and Elliott, Tom S.J. (2006). RAPID for the typing of coagulase-negative staphylococci implicated in catheter-related bloodstream infection. Journal of Infection, 52 (4), pp. 282-289. DOI 10.1016/j.jinf.2005.06.005

Keywords

  • central venous catheter-related bloodstream infection
  • coagulase-negative staphylococci
  • genotyping: random amplification of polymorphic DNA
  • pulsed-field gel electrophoresis

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