Rapid quantification and validation of lipid concentrations within liposomes

Carla B. Roces; Elisabeth Kastner; Peter Stone; Deborah Lowry; Yvonne Perrie

doi:10.3390/pharmaceutics8030029

Rapid quantification and validation of lipid concentrations within liposomes

Carla B. Roces
, Elisabeth Kastner
, Peter Stone
, Deborah Lowry
, Yvonne Perrie^*

^*Corresponding author for this work

University of Strathclyde
University of Ulster

Research output: Contribution to journal › Special issue › peer-review

36 Citations (SciVal)

137 Downloads (Pure)

Abstract

Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and D-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R² > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.

Original language	English
Article number	29
Journal	Pharmaceutics
Volume	8
Issue number	3
DOIs	https://doi.org/10.3390/pharmaceutics8030029
Publication status	Published - 13 Sept 2016

Bibliographical note

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Funding: EPSRC (EP/I033270/1); EU Horizon 2020 (643381); and the EPSRC and the BBSRC (Industrial Case Award BB/L017245/1).

Data can be found at DOI: 10.15129/4338bb40-6f84-4dde-b275-6f1656cc5c05.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cholesterol
ELSD
HPLC
lipids
liposomes
quantification

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10.3390/pharmaceutics8030029Licence: CC BY 3.0

Rapid quantification and validation of lipid concentrations within liposomes
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 1.23 MBLicence: CC BY 3.0

http://www.mdpi.com/1999-4923/8/3/29Licence: CC BY 3.0

Cite this

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title = "Rapid quantification and validation of lipid concentrations within liposomes",

abstract = "Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and D-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R2 > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.",

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AU - Roces, Carla B.

AU - Kastner, Elisabeth

AU - Stone, Peter

AU - Lowry, Deborah

AU - Perrie, Yvonne

N1 - © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). Funding: EPSRC (EP/I033270/1); EU Horizon 2020 (643381); and the EPSRC and the BBSRC (Industrial Case Award BB/L017245/1). Data can be found at DOI: 10.15129/4338bb40-6f84-4dde-b275-6f1656cc5c05.

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N2 - Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and D-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R2 > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.

AB - Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and D-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R2 > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.

KW - cholesterol

KW - ELSD

KW - HPLC

KW - lipids

KW - liposomes

KW - quantification

UR - https://www.scopus.com/pages/publications/84987790125

U2 - 10.3390/pharmaceutics8030029

DO - 10.3390/pharmaceutics8030029

M3 - Special issue

AN - SCOPUS:84987790125

SN - 1999-4923

VL - 8

JO - Pharmaceutics

JF - Pharmaceutics

IS - 3

M1 - 29

ER -

Rapid quantification and validation of lipid concentrations within liposomes

Abstract

Bibliographical note

UN SDGs

Keywords

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