Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy

A.L. Casey; T. Worthington; R.S. Bonser; P.A. Lambert; T.S.J. Elliott

doi:10.1016/j.jinf.2005.06.006

Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy

A.L. Casey, T. Worthington, R.S. Bonser, P.A. Lambert, T.S.J. Elliott

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: To determine the sensitivity and specificity of a novel ELISA for the serodiagnosis of surgical site infection (SSI) due to staphylococci following median sternotomy. Methods: Twelve patients with a superficial sternal SSI and 19 with a deep sternal SSI due to Staphylococcus aureus were compared with 37 control patients who also underwent median sternotomy for cardiac surgery but exhibited no microbiological or clinical symptoms of infection. A further five patients with sternal SSI due to coagulase-negative (CoNS) staphylococci were studied. An ELISA incorporating a recently recognised exocellular short chain form of lipoteichoic acid (lipid S) recovered from CoNS, was used to determine serum levels of anti-lipid S IgG in all patient groups. Results: Serum anti-lipid S IgG titres of patients with sternal SSI due to S. aureus were significantly higher than the control patients (P<0.0001). In addition, patients with deep sternal SSI had significantly higher serum anti-lipid S IgG titres than patients with superficial sternal SSI (P=0.03). Serum anti-lipid S IgG titres of patients with sternal SSI due to CoNS were significantly higher than the control patients (P=0.001). Conclusion: The lipid S ELISA may facilitate the diagnosis of sternal SSI due to S. aureus and could also be of value with infection due to CoNS. © 2005 Published by Elsevier Ltd. on behalf of The Bristish Infection Society.

Original language	English
Pages (from-to)	276-281
Number of pages	6
Journal	Journal of Infection
Volume	52
Issue number	4
DOIs	https://doi.org/10.1016/j.jinf.2005.06.006
Publication status	Published - Apr 2006

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Casey, A.L.; Worthington, Tony; Bonser, R.S.; Lambert, Peter A. and Elliott, Tom S.J. (2006). Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy. Journal of Infection, 52 (4), pp. 276-281. DOI 10.1016/j.jinf.2005.06.006

Keywords

ELISA
S. aureus
sternotomy
surgical site infection

Access to Document

10.1016/j.jinf.2005.06.006

Rapid serodiagnosis of Staphylococcus aureus surgical site infection
NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Casey, A.L.; Worthington, Tony; Bonser, R.S.; Lambert, Peter A. and Elliott, Tom S.J. (2006). Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy. Journal of Infection, 52 (4), pp. 276-281. DOI 10.1016/j.jinf.2005.06.006

Cite this

@article{cabc3fbbffca43f198806e2c37fa3c09,

title = "Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy",

abstract = "Objectives: To determine the sensitivity and specificity of a novel ELISA for the serodiagnosis of surgical site infection (SSI) due to staphylococci following median sternotomy. Methods: Twelve patients with a superficial sternal SSI and 19 with a deep sternal SSI due to Staphylococcus aureus were compared with 37 control patients who also underwent median sternotomy for cardiac surgery but exhibited no microbiological or clinical symptoms of infection. A further five patients with sternal SSI due to coagulase-negative (CoNS) staphylococci were studied. An ELISA incorporating a recently recognised exocellular short chain form of lipoteichoic acid (lipid S) recovered from CoNS, was used to determine serum levels of anti-lipid S IgG in all patient groups. Results: Serum anti-lipid S IgG titres of patients with sternal SSI due to S. aureus were significantly higher than the control patients (P<0.0001). In addition, patients with deep sternal SSI had significantly higher serum anti-lipid S IgG titres than patients with superficial sternal SSI (P=0.03). Serum anti-lipid S IgG titres of patients with sternal SSI due to CoNS were significantly higher than the control patients (P=0.001). Conclusion: The lipid S ELISA may facilitate the diagnosis of sternal SSI due to S. aureus and could also be of value with infection due to CoNS. {\textcopyright} 2005 Published by Elsevier Ltd. on behalf of The Bristish Infection Society.",

keywords = "ELISA, S. aureus, sternotomy, surgical site infection",

author = "A.L. Casey and T. Worthington and R.S. Bonser and P.A. Lambert and T.S.J. Elliott",

note = "NOTICE: this is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Casey, A.L.; Worthington, Tony; Bonser, R.S.; Lambert, Peter A. and Elliott, Tom S.J. (2006). Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy. Journal of Infection, 52 (4), pp. 276-281. DOI 10.1016/j.jinf.2005.06.006",

year = "2006",

month = apr,

doi = "10.1016/j.jinf.2005.06.006",

language = "English",

volume = "52",

pages = "276--281",

journal = "Journal of Infection",

issn = "0163-4453",

publisher = "W.B. Saunders Ltd",

number = "4",

}

TY - JOUR

T1 - Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy

AU - Casey, A.L.

AU - Worthington, T.

AU - Bonser, R.S.

AU - Lambert, P.A.

AU - Elliott, T.S.J.

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Casey, A.L.; Worthington, Tony; Bonser, R.S.; Lambert, Peter A. and Elliott, Tom S.J. (2006). Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy. Journal of Infection, 52 (4), pp. 276-281. DOI 10.1016/j.jinf.2005.06.006

PY - 2006/4

Y1 - 2006/4

N2 - Objectives: To determine the sensitivity and specificity of a novel ELISA for the serodiagnosis of surgical site infection (SSI) due to staphylococci following median sternotomy. Methods: Twelve patients with a superficial sternal SSI and 19 with a deep sternal SSI due to Staphylococcus aureus were compared with 37 control patients who also underwent median sternotomy for cardiac surgery but exhibited no microbiological or clinical symptoms of infection. A further five patients with sternal SSI due to coagulase-negative (CoNS) staphylococci were studied. An ELISA incorporating a recently recognised exocellular short chain form of lipoteichoic acid (lipid S) recovered from CoNS, was used to determine serum levels of anti-lipid S IgG in all patient groups. Results: Serum anti-lipid S IgG titres of patients with sternal SSI due to S. aureus were significantly higher than the control patients (P<0.0001). In addition, patients with deep sternal SSI had significantly higher serum anti-lipid S IgG titres than patients with superficial sternal SSI (P=0.03). Serum anti-lipid S IgG titres of patients with sternal SSI due to CoNS were significantly higher than the control patients (P=0.001). Conclusion: The lipid S ELISA may facilitate the diagnosis of sternal SSI due to S. aureus and could also be of value with infection due to CoNS. © 2005 Published by Elsevier Ltd. on behalf of The Bristish Infection Society.

AB - Objectives: To determine the sensitivity and specificity of a novel ELISA for the serodiagnosis of surgical site infection (SSI) due to staphylococci following median sternotomy. Methods: Twelve patients with a superficial sternal SSI and 19 with a deep sternal SSI due to Staphylococcus aureus were compared with 37 control patients who also underwent median sternotomy for cardiac surgery but exhibited no microbiological or clinical symptoms of infection. A further five patients with sternal SSI due to coagulase-negative (CoNS) staphylococci were studied. An ELISA incorporating a recently recognised exocellular short chain form of lipoteichoic acid (lipid S) recovered from CoNS, was used to determine serum levels of anti-lipid S IgG in all patient groups. Results: Serum anti-lipid S IgG titres of patients with sternal SSI due to S. aureus were significantly higher than the control patients (P<0.0001). In addition, patients with deep sternal SSI had significantly higher serum anti-lipid S IgG titres than patients with superficial sternal SSI (P=0.03). Serum anti-lipid S IgG titres of patients with sternal SSI due to CoNS were significantly higher than the control patients (P=0.001). Conclusion: The lipid S ELISA may facilitate the diagnosis of sternal SSI due to S. aureus and could also be of value with infection due to CoNS. © 2005 Published by Elsevier Ltd. on behalf of The Bristish Infection Society.

KW - ELISA

KW - S. aureus

KW - sternotomy

KW - surgical site infection

UR - http://www.scopus.com/inward/record.url?scp=33645283338&partnerID=8YFLogxK

U2 - 10.1016/j.jinf.2005.06.006

DO - 10.1016/j.jinf.2005.06.006

M3 - Article

C2 - 16045994

SN - 0163-4453

VL - 52

SP - 276

EP - 281

JO - Journal of Infection

JF - Journal of Infection

IS - 4

ER -

Rapid serodiagnosis of Staphylococcus aureus surgical site infection following median sternotomy

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this