Reactive oxygen species induce antigenic changes in DNA

S. Blount, Helen R. Griffiths, Joseph Lunec

Research output: Contribution to journalArticlepeer-review


Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.
Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalFEBS Letters
Issue number1-2
Publication statusPublished - 13 Mar 1989


  • reactive oxygen species
  • DNA antibody
  • anti-
  • systemic lupus erythematosus


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