Reactive oxygen species induce antigenic changes in DNA

S. Blount, Helen R. Griffiths, Joseph Lunec

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.
Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalFEBS Letters
Volume245
Issue number1-2
DOIs
Publication statusPublished - 13 Mar 1989

Fingerprint

Reactive Oxygen Species
DNA
Hydroxyl Radical
Immunoglobulin A
Respiratory Burst
Antinuclear Antibodies
Systemic Lupus Erythematosus
Metal ions
Immunoglobulin M
Immunoglobulin G
Metals
Ions
Inflammation
Antibodies
Serum
Experiments

Keywords

  • reactive oxygen species
  • DNA antibody
  • anti-
  • systemic lupus erythematosus

Cite this

Blount, S. ; Griffiths, Helen R. ; Lunec, Joseph. / Reactive oxygen species induce antigenic changes in DNA. In: FEBS Letters. 1989 ; Vol. 245, No. 1-2. pp. 100-104.
@article{36f408968b084ce585778dbd33d1b9f7,
title = "Reactive oxygen species induce antigenic changes in DNA",
abstract = "Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.",
keywords = "reactive oxygen species, DNA antibody, anti-, systemic lupus erythematosus",
author = "S. Blount and Griffiths, {Helen R.} and Joseph Lunec",
year = "1989",
month = "3",
day = "13",
doi = "10.1016/0014-5793(89)80200-5",
language = "English",
volume = "245",
pages = "100--104",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

Blount, S, Griffiths, HR & Lunec, J 1989, 'Reactive oxygen species induce antigenic changes in DNA', FEBS Letters, vol. 245, no. 1-2, pp. 100-104. https://doi.org/10.1016/0014-5793(89)80200-5

Reactive oxygen species induce antigenic changes in DNA. / Blount, S.; Griffiths, Helen R.; Lunec, Joseph.

In: FEBS Letters, Vol. 245, No. 1-2, 13.03.1989, p. 100-104.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reactive oxygen species induce antigenic changes in DNA

AU - Blount, S.

AU - Griffiths, Helen R.

AU - Lunec, Joseph

PY - 1989/3/13

Y1 - 1989/3/13

N2 - Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.

AB - Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.

KW - reactive oxygen species

KW - DNA antibody

KW - anti-

KW - systemic lupus erythematosus

UR - https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2889%2980200-5

U2 - 10.1016/0014-5793(89)80200-5

DO - 10.1016/0014-5793(89)80200-5

M3 - Article

VL - 245

SP - 100

EP - 104

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -