Abstract
Original language | English |
---|---|
Pages (from-to) | 100-104 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 245 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 13 Mar 1989 |
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Keywords
- reactive oxygen species
- DNA antibody
- anti-
- systemic lupus erythematosus
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Reactive oxygen species induce antigenic changes in DNA. / Blount, S.; Griffiths, Helen R.; Lunec, Joseph.
In: FEBS Letters, Vol. 245, No. 1-2, 13.03.1989, p. 100-104.Research output: Contribution to journal › Article
TY - JOUR
T1 - Reactive oxygen species induce antigenic changes in DNA
AU - Blount, S.
AU - Griffiths, Helen R.
AU - Lunec, Joseph
PY - 1989/3/13
Y1 - 1989/3/13
N2 - Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.
AB - Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.
KW - reactive oxygen species
KW - DNA antibody
KW - anti-
KW - systemic lupus erythematosus
UR - https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2889%2980200-5
U2 - 10.1016/0014-5793(89)80200-5
DO - 10.1016/0014-5793(89)80200-5
M3 - Article
VL - 245
SP - 100
EP - 104
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -