Real-time detection of DNA interactions with long-period fiber-grating-based biosensor

Xianfeng Chen*, Lin Zhang, Kaiming Zhou, Edward Davies, Kate Sugden, Ian Bennion, Marcus D. Hughes, Anna V. Hine

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Using an optical biosensor based on a dual-peak long-period fiber grating, we have demonstrated the detection of interactions between biomolecules in real time. Silanization of the grating surface was successfully realized for the covalent immobilization of probe DNA, which was subsequently hybridized with the complementary target DNA sequence. It is interesting to note that the DNA biosensor was reusable after being stripped off the hybridized target DNA from the grating surface, demonstrating a function of multiple usability. © 2007 Optical Society of America.

Original languageEnglish
Pages (from-to)2541-2543
Number of pages3
JournalOptics Letters
Volume32
Issue number17
DOIs
Publication statusPublished - 20 Aug 2007

Fingerprint

bioinstrumentation
deoxyribonucleic acid
gratings
fibers
interactions
immobilization
probes

Bibliographical note

© 2007 Optical Society of America. This paper was published in Optics Letters and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-17-2541. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Keywords

  • biosensing techniques
  • DNA
  • equipment design
  • ebium
  • interferometry
  • lasers
  • light
  • models, theoretical
  • oscillometry
  • semiconductors
  • time factors
  • Ytterbium

Cite this

Chen, Xianfeng ; Zhang, Lin ; Zhou, Kaiming ; Davies, Edward ; Sugden, Kate ; Bennion, Ian ; Hughes, Marcus D. ; Hine, Anna V. / Real-time detection of DNA interactions with long-period fiber-grating-based biosensor. In: Optics Letters. 2007 ; Vol. 32, No. 17. pp. 2541-2543.
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Real-time detection of DNA interactions with long-period fiber-grating-based biosensor. / Chen, Xianfeng; Zhang, Lin; Zhou, Kaiming; Davies, Edward; Sugden, Kate; Bennion, Ian; Hughes, Marcus D.; Hine, Anna V.

In: Optics Letters, Vol. 32, No. 17, 20.08.2007, p. 2541-2543.

Research output: Contribution to journalArticle

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AU - Hughes, Marcus D.

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