Real time optical immunosensing with flow-through porous alumina membranes

Jesús Álvarez, Laura Sola, Marina Cretich, Marcus J. Swann, Kristinn B. Gylfasson, Tormod Volden, Marcella Chiari, Daniel Hill*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.

Original languageEnglish
Pages (from-to)834-839
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume202
DOIs
Publication statusPublished - 31 Oct 2014

Fingerprint

immunoassay
Aluminum Oxide
Assays
Lactoglobulins
Alumina
aluminum oxides
rabbits
membranes
Membranes
porosity
Allergens
bioassay
Polarimeters
Streptavidin
Bioassay
fluidics
polarimetry
Fluidics
Dynamic mechanical analysis
antibodies

Bibliographical note

© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Copolymer
  • Form birefringence
  • Optical biosensing
  • Polarimetry
  • Porous alumina
  • Quantum dots

Cite this

Álvarez, J., Sola, L., Cretich, M., Swann, M. J., Gylfasson, K. B., Volden, T., ... Hill, D. (2014). Real time optical immunosensing with flow-through porous alumina membranes. Sensors and Actuators, B: Chemical, 202, 834-839. https://doi.org/10.1016/j.snb.2014.06.027
Álvarez, Jesús ; Sola, Laura ; Cretich, Marina ; Swann, Marcus J. ; Gylfasson, Kristinn B. ; Volden, Tormod ; Chiari, Marcella ; Hill, Daniel. / Real time optical immunosensing with flow-through porous alumina membranes. In: Sensors and Actuators, B: Chemical. 2014 ; Vol. 202. pp. 834-839.
@article{5150268672b3495da8bf4d244191b15f,
title = "Real time optical immunosensing with flow-through porous alumina membranes",
abstract = "Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.",
keywords = "Copolymer, Form birefringence, Optical biosensing, Polarimetry, Porous alumina, Quantum dots",
author = "Jes{\'u}s {\'A}lvarez and Laura Sola and Marina Cretich and Swann, {Marcus J.} and Gylfasson, {Kristinn B.} and Tormod Volden and Marcella Chiari and Daniel Hill",
note = "{\circledC} 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/",
year = "2014",
month = "10",
day = "31",
doi = "10.1016/j.snb.2014.06.027",
language = "English",
volume = "202",
pages = "834--839",

}

Álvarez, J, Sola, L, Cretich, M, Swann, MJ, Gylfasson, KB, Volden, T, Chiari, M & Hill, D 2014, 'Real time optical immunosensing with flow-through porous alumina membranes', Sensors and Actuators, B: Chemical, vol. 202, pp. 834-839. https://doi.org/10.1016/j.snb.2014.06.027

Real time optical immunosensing with flow-through porous alumina membranes. / Álvarez, Jesús; Sola, Laura; Cretich, Marina; Swann, Marcus J.; Gylfasson, Kristinn B.; Volden, Tormod; Chiari, Marcella; Hill, Daniel.

In: Sensors and Actuators, B: Chemical, Vol. 202, 31.10.2014, p. 834-839.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Real time optical immunosensing with flow-through porous alumina membranes

AU - Álvarez, Jesús

AU - Sola, Laura

AU - Cretich, Marina

AU - Swann, Marcus J.

AU - Gylfasson, Kristinn B.

AU - Volden, Tormod

AU - Chiari, Marcella

AU - Hill, Daniel

N1 - © 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

PY - 2014/10/31

Y1 - 2014/10/31

N2 - Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.

AB - Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.

KW - Copolymer

KW - Form birefringence

KW - Optical biosensing

KW - Polarimetry

KW - Porous alumina

KW - Quantum dots

UR - http://www.scopus.com/inward/record.url?scp=84903649673&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/science/article/pii/S0925400514007163?via%3Dihub

U2 - 10.1016/j.snb.2014.06.027

DO - 10.1016/j.snb.2014.06.027

M3 - Article

AN - SCOPUS:84903649673

VL - 202

SP - 834

EP - 839

ER -

Álvarez J, Sola L, Cretich M, Swann MJ, Gylfasson KB, Volden T et al. Real time optical immunosensing with flow-through porous alumina membranes. Sensors and Actuators, B: Chemical. 2014 Oct 31;202:834-839. https://doi.org/10.1016/j.snb.2014.06.027