Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors

Cathryn Weston, Ian Winfield, Matthew Harris, Rose Hodgson, Archna Shah, Simon J. Dowell, Juan Carlos Mobarec, David A. Woodlock, Christopher A. Reynolds, David R. Poyner, Harriet A. Watkins, Graham Ladds*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The calcitonin gene-related peptide (CGRP) family of G protein- coupled receptors (GPCRs) is formed through the association of the calcitonin receptor-like receptor (CLR) and one of three receptor activity-modifying proteins (RAMPs). Binding of one of the three peptide ligands, CGRP, adrenomedullin (AM), and intermedin/adrenomedullin 2 (AM2), is well known to result in aGαs-mediated increase in cAMP. Here we used modified yeast strains that couple receptor activation to cell growth, via chimeric yeast/Gα subunits, and HEK-293 cells to characterize the effect of different RAMP and ligand combinations on this pathway. We not only demonstrate functional couplings to both Gαs and Gαq but also identify a Gαi component to CLR signaling in both yeast and HEK-293 cells, which is absent in HEK-293S cells. We show that the CGRP family of receptors displays both ligand- and RAMPdependent signaling bias among the Gαs, Gαi, and Gαq/11 pathways. The results are discussed in the context of RAMP interactions probed through molecular modeling and molecular dynamics simulations of the RAMP-GPCR-G protein complexes. This study further highlights the importance of RAMPs to CLR pharmacology and to bias in general, as well as identifying the importance of choosing an appropriate model system for the study of GPCR pharmacology.

Original languageEnglish
Pages (from-to)21925-21944
Number of pages20
JournalJournal of Biological Chemistry
Volume291
Issue number42
Early online date26 Aug 2016
DOIs
Publication statusPublished - 14 Oct 2016

Fingerprint

Receptor Activity-Modifying Proteins
Calcitonin Gene-Related Peptide Receptors
Calcitonin Gene-Related Peptide
GTP-Binding Proteins
Calcitonin Receptor-Like Protein
G-Protein-Coupled Receptors
Yeast
Adrenomedullin
Yeasts
HEK293 Cells
Ligands
Pharmacology
Molecular modeling
Cell growth
Molecular Dynamics Simulation
Molecular dynamics
Chemical activation
Association reactions
Peptides
Computer simulation

Bibliographical note

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons CC-BY license.

Funding: National Heart Foundation of New Zealand; University of Auckland seed fund; BBSRC (BB/M00015X/1, BB/M000176/1 and BB/M006883/1 and BBSRC Doctoral Training Partnership Grant BB/JO14540/1); MRC Doctoral Training Partnership MR/J003964/1; Warwick Impact Fund (RD13301) from the Warwick Research Development Fund and the Warwick Undergraduate Research Scholarship Scheme.

This article contains supplemental Movies 1 and 2.

Keywords

  • G protein-coupled receptor
  • GPCR
  • molecular dynamics
  • molecular modeling
  • signal transduction
  • yeast
  • adrenomedullin
  • adrenomedullin 2
  • RAMP
  • signal bias

Cite this

Weston, Cathryn ; Winfield, Ian ; Harris, Matthew ; Hodgson, Rose ; Shah, Archna ; Dowell, Simon J. ; Mobarec, Juan Carlos ; Woodlock, David A. ; Reynolds, Christopher A. ; Poyner, David R. ; Watkins, Harriet A. ; Ladds, Graham. / Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 42. pp. 21925-21944.
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Weston, C, Winfield, I, Harris, M, Hodgson, R, Shah, A, Dowell, SJ, Mobarec, JC, Woodlock, DA, Reynolds, CA, Poyner, DR, Watkins, HA & Ladds, G 2016, 'Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors', Journal of Biological Chemistry, vol. 291, no. 42, pp. 21925-21944. https://doi.org/10.1074/jbc.M116.751362

Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors. / Weston, Cathryn; Winfield, Ian; Harris, Matthew; Hodgson, Rose; Shah, Archna; Dowell, Simon J.; Mobarec, Juan Carlos; Woodlock, David A.; Reynolds, Christopher A.; Poyner, David R.; Watkins, Harriet A.; Ladds, Graham.

In: Journal of Biological Chemistry, Vol. 291, No. 42, 14.10.2016, p. 21925-21944.

Research output: Contribution to journalArticle

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AU - Weston, Cathryn

AU - Winfield, Ian

AU - Harris, Matthew

AU - Hodgson, Rose

AU - Shah, Archna

AU - Dowell, Simon J.

AU - Mobarec, Juan Carlos

AU - Woodlock, David A.

AU - Reynolds, Christopher A.

AU - Poyner, David R.

AU - Watkins, Harriet A.

AU - Ladds, Graham

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