Recombinant protein production in yeast: methods and protocols

Research output: Book/ReportAnthology

Abstract

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Original languageEnglish
PublisherHumana Press
Number of pages240
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Keywords

  • scale-up
  • bioreactor
  • Pichia pastoris
  • Saccharomyces cerevisiae

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  • Research Output

    • 7 Chapter (peer-reviewed)
    • 1 Foreword/postscript

    Large-scale production of secreted proteins in Pichia pastoris

    Bora, N., 25 Feb 2012, Recombinant protein production in yeast. Bill, R. M. (ed.). Humana Press, p. 217-235 19 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising yeast as a host for recombinant protein production (review)

    Bonander, N. & Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 1-9 9 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Cite this

    Bill, R. M. (Ed.) (2012). Recombinant protein production in yeast: methods and protocols. (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5