Recombinant protein production in yeast: methods and protocols

Roslyn M. Bill

doi:10.1007/978-1-61779-770-5

Recombinant protein production in yeast: methods and protocols

Research output: Book/Report › Edited Book

Abstract

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Original language	English
Publisher	Humana Press
Number of pages	240
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5
Publication status	Published - 25 Feb 2012

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Keywords

scale-up
bioreactor
Pichia pastoris
Saccharomyces cerevisiae

Access to Document

10.1007/978-1-61779-770-5

Cite this

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Recombinant protein production in yeast: methods and protocols

Abstract

Publication series

Keywords

Access to Document

Other files and links

Fingerprint

Large-scale production of secreted proteins in Pichia pastoris

Optimising Pichia pastoris induction

Optimising yeast as a host for recombinant protein production (review)

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